CDK5 regulatory subunit-associated protein 2; CDK5 activator-binding protein C48; Centrosome-associated protein 215; CDK5RAP2; CEP215; KIAA1633

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgM

527CT22.9.2

Mouse

This CDK5RAP2 Antibody is generated from mouse immunized with recombinant protein of human CDK5RAP2.

Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CDK5RAP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. The encoded protein may also be a substrate of neuronal CDC2-like kinase. Multiple transcript variants exist for this gene, but the full-length nature of only two has been determined.

Cell Biology; Neuroscience; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:500~2000

CDK5RAP2 Antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CDK5RAP2 antibody detected the CDK5RAP2 protein (arrow).

215038

CDK5 regulatory subunit associated protein 2

CDK5 regulatory subunit-associated protein 2

CDK5 regulatory subunit-associated protein 2

CEP215; KIAA1633??[Similar Products]

CK5P2_HUMAN

This gene encodes a regulator of CDK5 (cyclin-dependent kinase 5) activity. The protein encoded by this gene is localized to the centrosome and Golgi complex, interacts with CDK5R1 and pericentrin (PCNT), plays a role in centriole engagement and microtubule nucleation, and has been linked to primary microcephaly and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

CDK5RAP2: Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation. Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3). A disorder defined as a head circumference more than 3 standard deviations below the age- related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: spindle pole; Golgi apparatus; centrosome; microtubule; pericentriolar material; cytoskeleton; perinuclear region of cytoplasm; cytoplasm; cell junction; cytosol

Molecular Function: calmodulin binding; tubulin binding; protein binding; microtubule binding; protein kinase binding

Biological Process: regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of centriole replication; chromosome segregation; negative regulation of neuron differentiation; establishment of mitotic spindle orientation; neurogenesis; centrosome organization and biogenesis; mitotic cell cycle; brain development; G2/M transition of mitotic cell cycle; microtubule bundle formation

Disease: Microcephaly 3, Primary, Autosomal Recessive

Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Wang, Z., et al. J. Biol. Chem. 285(29):22658-22665(2010)
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Lee, S., et al. Cell Cycle 9(4):774-783(2010)
Barber, M.J., et al. PLoS ONE 5 (3), E9763 (2010)

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