Product Name
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL), Recombinant Protein
Full Product Name
Recombinant Human 3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase, Sf9
Product Synonym Names
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase; 3-Hydroxymethyl-3-Methylglutaryl-Coenzyme A Lyase; 3-Hydroxy-3-Methylglutarate-CoA Lyase; Hydroxymethylglutaricaciduria; HMG-CoA Lyase; EC 4.1.3.4; HL; Mitochondrial 3-Hydroxy-3-Methylglutaryl-CoA Lyase; Hydroxymethylglutaryl-CoA Lyase, mitochondrial; 3-Hydroxy-3-Methylglutaryl-CoA Lyase; Hydroxymethylglutaryl-CoA lyase, mitochondrial; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; HMGCL Human, Sf9; 3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase Human Recombinant, Sf9
Product Gene Name
HMGCL recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
M TLPKRVKIV EVGPRDGLQN EKNIVSTPVK IKLIDMLSEA GLSVIETTSF VSPKWVPQMG DHTEVLKGIQ KFPGINYPVL TPNLKGFEAA VAAGAKEVVI FGAASELFTK KNINCSIEES FQRFDAILKA AQSANISVRG YVSCALGCPY EGKISPAKVA EVTKKFYSMG CYEISLGDTI GVGTPGIMKD MLSAVMQEVP LAALAVHCHD TYGQALANTL MALQMGVSVV DSSVAGLGGC PYAQGASGNL ATEDLVYMLE GLGIHTGVNL QKLLEAGNFI CQALNRKTSS KVAQATCKLH HHHHH.
3D Structure
ModBase 3D Structure for P35914
Host
Sf9, Baculovirus cells
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE
Form/Format
Sterile filtered colorless solution
Protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4), 20% glycerol and 1mM DTT
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles
Other Notes
Small volumes of HMGCL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HMGCL recombinant protein
Description: HMGCL Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 305 amino acids (28-325 a.a.) and having a molecular mass of 32.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). HMGCL is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction: Hydroxymethylglutaryl-CoA lyase (HMGCL) is a mitochondrial matrix protein which is a member of the HMG-CoA lyase family. HMGCL is a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies which, during fasting, provides a major source of energy for the heart, brain and kidney. More precisely, HMGCL catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA.
Product Categories/Family for HMGCL recombinant protein
ENZYMES; Enzymes; Lyase; HMGCL Human, Sf9
NCBI/Uniprot data below describe general gene information for HMGCL. It may not necessarily be applicable to this product.
NCBI Accession #
P35914.2
[Other Products]
UniProt Primary Accession #
P35914
[Other Products]
UniProt Secondary Accession #
Q6IBC0; Q96FP8; B4DUP4; B7UCC6; D3Y5K7[Other Products]
UniProt Related Accession #
P35914[Other Products]
Molecular Weight
20,222 Da
NCBI Official Full Name
Hydroxymethylglutaryl-CoA lyase, mitochondrial
NCBI Official Synonym Full Names
3-hydroxymethyl-3-methylglutaryl-CoA lyase
NCBI Official Symbol
HMGCL??[Similar Products]
NCBI Official Synonym Symbols
HL
??[Similar Products]
NCBI Protein Information
hydroxymethylglutaryl-CoA lyase, mitochondrial
UniProt Protein Name
Hydroxymethylglutaryl-CoA lyase, mitochondrial
UniProt Synonym Protein Names
3-hydroxy-3-methylglutarate-CoA lyase
Protein Family
3-hydroxymethyl-3-methylglutaryl-CoA lyase
UniProt Gene Name
HMGCL??[Similar Products]
UniProt Synonym Gene Names
HL; HMG-CoA lyase??[Similar Products]
NCBI Summary for HMGCL
The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Comments for HMGCL
Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.
Research Articles on HMGCL
1. Data suggest that HMGCS1 (HMG-CoA synthase 1) signals through ketogenesis/acetoacetate to promote cell proliferation and BRAF(V600E)-dependent MEK1 activation in BRAF(V600E)-positive melanoma and colon cancer cells; HMGCS1 co-localizes with HMGCL (HMG-CoA lyase) and BRAF(V600E) in cytosol of melanoma and colon cancer cells. (BRAF = proto-oncogene protein B-raf)
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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