MLL2; myeloid/lymphoid or mixed-lineage leukemia 2; ALR; ALL1-related gene; AAD10; CAGL114; KMT2B; MLL4; TNRC21; ALL1-related; trinucleotide repeat containing 21; MLL2 antibody; myeloid/lymphoid or mixed-lineage leukemia 2 antibody; ALR antibody; ALL1-related gene antibody; AAD10 antibody; CAGL114 antibody; KMT2B antibody; MLL4 antibody; TNRC21 antibody; ALL1-related antibody; trinucleotide repeat containing 21 antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-MLL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Placenta shows nuclear stainng in the trophoblasts. Similar nuclear patches are observed in Human Liver. Recommended concentration, 4-8ug/ml.
Western Blot: Preliminary experiments gave bands at approx 75kDa and 25kDa in lysates of cell line K562 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 593kDa according to NP_003473.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

(4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

(3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

593,677 Da

lysine methyltransferase 2D

histone-lysine N-methyltransferase 2D

Histone-lysine N-methyltransferase 2D

ALR; MLL2; MLL4; Lysine N-methyltransferase 2D??[Similar Products]

KMT2D_HUMAN

The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. [provided by RefSeq, Oct 2010]

MLL4: Histone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Defects in MLL2 are the cause of Kabuki syndrome type 1 (KABUK1). A congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Methyltransferase, protein lysine; Nuclear receptor co-regulator; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: histone methyltransferase complex; nucleoplasm; nucleus

Molecular Function: DNA binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding; zinc ion binding

Biological Process: chromatin silencing; histone H3-K4 methylation; oocyte growth; oogenesis; positive regulation of cell proliferation; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to estrogen stimulus; transcription, DNA-dependent

Disease: Kabuki Syndrome 1

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