Product Name
Thymidine Phosphorylase (TP), ELISA Kit
Full Product Name
Thymidine Phosphorylase (TP) ELISA Kit
Product Synonym Names
TYMP; PDECGF; ECGF1; MNGIE; TP; HPD-ECGF; TdRPase; Platelet-Derived Endothelial Cell Growth Factor; Gliostatin
Product Gene Name
TP elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
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3D Structure
ModBase 3D Structure for P19971
Specificity
This assay has high sensitivity and excellent specificity for detection of Thymidine Phosphorylase (TP).
No significant cross-reactivity or interference between Thymidine Phosphorylase (TP) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
1.56-100ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Thymidine Phosphorylase (TP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Thymidine Phosphorylase (TP) were tested on 3 different plates, 8 replicates in each plate.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of TP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for TP purchase
MBS2022163 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thymidine Phosphorylase (TP) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TP. The ELISA analytical biochemical technique of the MBS2022163 kit is based on TP antibody-TP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
TP elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidine Phosphorylase (TP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidine Phosphorylase (TP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidine Phosphorylase (TP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymidine Phosphorylase (TP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for TP elisa kit
Enzyme & Kinase; Tumor immunity
Typical Testing Data/Standard Curve (for reference only) of TP elisa kit
NCBI/Uniprot data below describe general gene information for TP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001107228.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001113756.2
[Other Products]
UniProt Primary Accession #
P19971
[Other Products]
UniProt Secondary Accession #
Q13390; Q8WVB7; A8MW15; H9KVA0[Other Products]
UniProt Related Accession #
P19971[Other Products]
Molecular Weight
50,405 Da
NCBI Official Full Name
thymidine phosphorylase isoform 1 proprotein
NCBI Official Synonym Full Names
thymidine phosphorylase
NCBI Official Symbol
TYMP??[Similar Products]
NCBI Official Synonym Symbols
TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
??[Similar Products]
NCBI Protein Information
thymidine phosphorylase
UniProt Protein Name
Thymidine phosphorylase
UniProt Synonym Protein Names
Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase
Protein Family
Trehalose phosphorylase
UniProt Gene Name
TYMP??[Similar Products]
UniProt Synonym Gene Names
ECGF1; TP; PD-ECGF??[Similar Products]
UniProt Entry Name
TYPH_HUMAN
NCBI Summary for TP
This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
UniProt Comments for TP
ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Protein type: Cytokine; Motility/polarity/chemotaxis; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Phosphorylase; Nucleotide Metabolism - pyrimidine; DNA replication; EC 2.4.2.4
Chromosomal Location of Human Ortholog: 22q13.33
Cellular Component: cytosol
Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding
Biological Process: mitochondrial genome maintenance; pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; cell differentiation; DNA replication; chemotaxis
Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)
Research Articles on TP
1. action of thymidine phosphorylase in non-small cell lung cancer: crosstalk with Nrf2 and HO-1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.