AQP1; AQP 1; AQP CHIP; AQP-1; AQP1; AQP1_HUMAN; Aquaporin CHIP; Aquaporin-1; Aquaporin-CHIP; Aquaporin1; Channel forming integral protein 28kDa; Channel like integral membrane protein 28 kDa; CHIP 28; CHIP28; CO; Colton blood group; Growth factor induced delayed early response protein; MGC26324; Urine water channel; Water channel protein CHIP 29; Water channel protein CHIP29; Water channel protein for red blood cells and kidney proximal tubule

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

1mg/mL (lot specific)

4 degree C

Small volumes of anti-Aqp1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background Info: Detects ~28.5kDa. May detect larger glycosylated bands ~35-50kDa.

Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin (3).

Ion Channels and Transporters

Western Blot (WB), Immunofluorescence (IF)

WB 1:1000-4000, IF: 1:200-400

Immunohistochemistry analysis using Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502). Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502) at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 um cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 1 protein using Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502). Primary Antibody: Rabbit Anti-Aquaporin 1 Polyclonal Antibody (SPC-502) at 1:2000. Showing glycosylated and non-glycosylated bands.

28,857 Da[Similar Products]

aquaporin 1

aquaporin-1; AQP-1; aquaporin-CHIP; Aquaporin 1 (aquaporin channel forming integral protein 28 (CHIP)); water channel protein for red blood cells and kidney proximal tubule

Aquaporin-1

Chip28; AQP-1??[Similar Products]

AQP1_RAT

integral membrane protein that is a major water transport molecule in the kidney proximal tubule and red blood cells [RGD, Feb 2006]

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, aquaporin family; Membrane protein, integral

Cellular Component: neuron projection; nuclear membrane; basolateral plasma membrane; brush border membrane; integral to plasma membrane; cell; integral to membrane; membrane; apical part of cell; axon; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; nerve terminal; nucleus; brush border; sarcolemma

Molecular Function: protein binding; potassium channel activity; ephrin receptor binding; water transporter activity; ammonium transmembrane transporter activity; intracellular cGMP activated cation channel activity; potassium ion transmembrane transporter activity; glycerol transmembrane transporter activity; water channel activity; transmembrane transporter activity; nitric oxide transporter activity; glycerol channel activity

Biological Process: glycerol transport; cGMP biosynthetic process; cerebrospinal fluid secretion; wound healing; water transport; carbon dioxide transport; response to hormone stimulus; negative regulation of caspase activity; sensory perception of pain; pancreatic juice secretion; positive regulation of saliva secretion; water homeostasis; odontogenesis; positive regulation of fibroblast proliferation; hyperosmotic salinity response; nitric oxide transport; multicellular organismal water homeostasis; cellular homeostasis; lateral ventricle development; lipid digestion; potassium ion transport; secretion by cell; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; hyperosmotic response; positive regulation of angiogenesis; renal water transport; camera-type eye morphogenesis; response to estrogen stimulus; adrenocorticotropin hormone secretion; establishment and/or maintenance of actin cytoskeleton polarity; cell volume homeostasis; negative regulation of apoptosis; positive regulation of cell migration

1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396. 2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258. 3. www.vivo.colostate.edu/hbooks/molecules/aqu aporins.html

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