Carboxypeptidase N catalytic chain; CPN; Anaphylatoxin inactivator; Arginine carboxypeptidase; Carboxypeptidase N polypeptide 1; Carboxypeptidase N small subunit; Kininase-1; Lysine carboxypeptidase; Plasma carboxypeptidase B; Serum carboxypeptidase N; SCPN; CPN1; ACBP

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This CPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-240 amino acids from the Central region of human CPN1.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 2 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CPN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Carboxypeptidase N is a plasma metallo-protease that
cleaves basic amino acids from the C terminal of peptides and
proteins. The enzyme is important in the regulation of peptides
like kinins and anaphylatoxins, and has also been known as
kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer
comprised of two identical regulatory subunits and two identical
catalytic subunits; this gene encodes the catalytic subunit.
Mutations in this gene can be associated with angioedema or chronic
urticaria resulting from carboxypeptidase N deficiency. [provided
by RefSeq].

Cell Biology

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

WB~~1:1000

Western blot analysis of CPN1 (arrow) using rabbit polyclonal CPN1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CPN1 gene.

CPN1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPN1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

CPN1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

52286

carboxypeptidase N, polypeptide 1

carboxypeptidase N catalytic chain

Carboxypeptidase N catalytic chain

ACBP; CPN; SCPN??[Similar Products]

CBPN_HUMAN

Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]

CPN1: Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation. Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND). Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder. Belongs to the peptidase M14 family.

Protein type: Secreted, signal peptide; Protease; Secreted; EC 3.4.17.3

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to glucocorticoid stimulus; proteolysis

Disease: Carboxypeptidase N Deficiency

Speliotes, E.K., et al. Hepatology 52(3):904-912(2010)
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Du, X.Y., et al. J. Biol. Chem. 284(2):751-758(2009)
Yuan, X., et al. Am. J. Hum. Genet. 83(4):520-528(2008)
Skidgel, R.A., et al. Int. Immunopharmacol. 7(14):1888-1899(2007)

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