Monoclonal ANAPC2; Anti-ANAPC2; Anaphase promoting complex subunit 2; APC2; KIAA1406; Cyclosome subunit 2; ANAPC2; ANAPC 2; ANAPC-2

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

1H8

Mouse

ANAPC2 antibody was purified by affinity chromatography.

Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.

500 ug-1 mg/ml (lot specific)

Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Small volumes of anti-ANAPC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse monoclonal ANAPC2 antibody

Cell Biology

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)

FC: 1:100
IF: 1:100
WB: 1:500-2000

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANAPC2 protein (Right) or empty vector (Left) detected with ANAPC2 antibody

Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANAPC2 protein using ANAPC2 antibody

93,469 Da

anaphase promoting complex subunit 2

anaphase-promoting complex subunit 2

Anaphase-promoting complex subunit 2

APC2; KIAA1406; APC2??[Similar Products]

ANC2_HUMAN

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008]

APC2: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the cullin family. The APC/C is composed of at least 12 subunits. Interacts through the cullin domain with ANAPC11 and with UBCH10. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol

Molecular Function: ubiquitin protein ligase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of axon extension; mitotic cell cycle spindle assembly checkpoint; positive regulation of synaptic plasticity; positive regulation of dendrite morphogenesis; mitotic cell cycle

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