Product Name
ANAPC2, siRNA
Full Product Name
ANAPC2 siRNA (Human)
Product Synonym Names
APC2; KIAA1406; Anaphase-promoting complex subunit 2; APC2; Cyclosome subunit 2
Product Gene Name
ANAPC2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UJX6
Specificity
ANAPC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ANAPC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ANAPC2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ANAPC2 sirna
siRNA to inhibit ANAPC2 expression using RNA interference
Applications Tested/Suitable for ANAPC2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ANAPC2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037498.1
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NCBI GenBank Nucleotide #
NM_013366.3
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UniProt Primary Accession #
Q9UJX6
[Other Products]
UniProt Secondary Accession #
Q5VSG1; Q96DG5; Q96GG4; Q9P2E1[Other Products]
UniProt Related Accession #
Q9UJX6[Other Products]
Molecular Weight
93,469 Da
NCBI Official Full Name
anaphase-promoting complex subunit 2
NCBI Official Synonym Full Names
anaphase promoting complex subunit 2
NCBI Official Symbol
ANAPC2??[Similar Products]
NCBI Official Synonym Symbols
APC2
??[Similar Products]
NCBI Protein Information
anaphase-promoting complex subunit 2
UniProt Protein Name
Anaphase-promoting complex subunit 2
UniProt Synonym Protein Names
Cyclosome subunit 2
Protein Family
Anaphase-promoting complex
UniProt Gene Name
ANAPC2??[Similar Products]
UniProt Synonym Gene Names
APC2; KIAA1406; APC2??[Similar Products]
UniProt Entry Name
ANC2_HUMAN
NCBI Summary for ANAPC2
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008]
UniProt Comments for ANAPC2
APC2: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the cullin family. The APC/C is composed of at least 12 subunits. Interacts through the cullin domain with ANAPC11 and with UBCH10. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol
Molecular Function: ubiquitin protein ligase binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of axon extension; mitotic cell cycle spindle assembly checkpoint; positive regulation of synaptic plasticity; mitotic cell cycle; positive regulation of dendrite morphogenesis
Research Articles on ANAPC2
1. The regulation of Mdm2 by the E3 ubiquitin ligase APC/C is shown. It has important therapeutic implications for tumors with Mdm2 overexpression.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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