Full Product Name
LIFR siRNA (Human)
Product Synonym Names
Leukemia inhibitory factor receptor; LIF receptor; LIF-R; CD antigen CD118
Product Gene Name
LIFR sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P42702
Specificity
LIFR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LIFR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LIFR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LIFR sirna
siRNA to inhibit LIFR expression using RNA interference
Applications Tested/Suitable for LIFR sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LIFR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001121143.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001127671.1
[Other Products]
UniProt Primary Accession #
P42702
[Other Products]
UniProt Secondary Accession #
Q6LCD9[Other Products]
UniProt Related Accession #
P42702[Other Products]
NCBI Official Full Name
leukemia inhibitory factor receptor
NCBI Official Synonym Full Names
leukemia inhibitory factor receptor alpha
NCBI Official Symbol
LIFR??[Similar Products]
NCBI Official Synonym Symbols
SWS; SJS2; STWS; CD118; LIF-R
??[Similar Products]
NCBI Protein Information
leukemia inhibitory factor receptor
UniProt Protein Name
Leukemia inhibitory factor receptor
UniProt Synonym Protein Names
CD_antigen: CD118
Protein Family
Leukemia inhibitory factor receptor
UniProt Gene Name
LIFR??[Similar Products]
UniProt Synonym Gene Names
LIF receptor; LIF-R??[Similar Products]
UniProt Entry Name
LIFR_HUMAN
NCBI Summary for LIFR
This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the ***** and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for LIFR
LIFR: Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the
biological activity of LIF by blocking its binding to receptors on target cells. Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS); also knowns as Schwartz-Jampel syndrome type 2 (SJS2). SWS is a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias. SWS is characterized by bowing of the lower limbs, with internal cortical thickening, wide metaphyses with abnormal trabecular pattern, and camptodactyly. Additional features include feeding and swallowing difficulties, as well as respiratory distress and hyperthermic episodes, which cause death in the first months of life. The rare survivors develop progressive scoliosis, spontaneous fractures, bowing of the lower limbs, with prominent joints and dysautonomia symptoms, including temperature instability, absent corneal and patellar reflexes, and smooth tongue. A chromosomal aberration involving LIFR is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(5;8)(p13;q12) with PLAG1. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5p13-p12
Cellular Component: integral to plasma membrane; receptor complex
Molecular Function: leukemia inhibitory factor receptor activity; oncostatin-M receptor activity; growth factor binding; ciliary neurotrophic factor receptor activity; ciliary neurotrophic factor receptor binding
Biological Process: cell surface receptor linked signal transduction; response to cytokine stimulus; cytokine and chemokine mediated signaling pathway; leukemia inhibitory factor signaling pathway; positive regulation of cell proliferation
Disease: Stuve-wiedemann Syndrome
Research Articles on LIFR
1. Studied the association of tubal pregnancy with leukemia inhibitory factor (LIF) and leukemia inhibitory factor receptor (LIFR) expression in oviduct tissues.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
