Full Product Name
JUP siRNA (Mouse)
Product Synonym Names
Junction plakoglobin; Desmoplakin III; Desmoplakin-3
Product Gene Name
JUP sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q02257
Specificity
JUP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse JUP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of JUP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
JUP sirna
siRNA to inhibit JUP expression using RNA interference
Applications Tested/Suitable for JUP sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for JUP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034723.1
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NCBI GenBank Nucleotide #
NM_010593.2
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UniProt Primary Accession #
Q02257
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UniProt Secondary Accession #
Q8CGD3[Other Products]
UniProt Related Accession #
Q02257[Other Products]
Molecular Weight
81,801 Da
NCBI Official Full Name
junction plakoglobin
NCBI Official Synonym Full Names
junction plakoglobin
NCBI Official Symbol
Jup??[Similar Products]
NCBI Official Synonym Symbols
PG; Ctnng; D930025P04Rik
??[Similar Products]
NCBI Protein Information
junction plakoglobin
UniProt Protein Name
Junction plakoglobin
UniProt Synonym Protein Names
Desmoplakin III; Desmoplakin-3
Protein Family
Junction plakoglobin
UniProt Gene Name
Jup??[Similar Products]
UniProt Entry Name
PLAK_MOUSE
UniProt Comments for JUP
CTNNG: Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Homodimer. Component of an E-cadherin/ catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma- catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1. Interacts with PTPRJ. Interacts with DSC2. Belongs to the beta-catenin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Cellular Component: desmosome; internal side of plasma membrane; focal adhesion; apicolateral plasma membrane; intermediate filament; fascia adherens; intercellular junction; catenin complex; cytosol; Z disc; actin cytoskeleton; cell-cell adherens junction; cytoskeleton; membrane; cytoplasm; plasma membrane; nucleus; cell junction; lateral plasma membrane
Molecular Function: signal transducer activity; protein binding; cadherin binding; transcription coactivator activity; structural molecule activity; protein kinase binding; protein phosphatase binding; alpha-catenin binding
Biological Process: skin development; cell migration; cell-cell adhesion; positive regulation of protein import into nucleus; protein heterooligomerization; regulation of cell fate specification; detection of mechanical stimulus; positive regulation of transcription factor activity; cell adhesion; regulation of cell proliferation
Research Articles on JUP
1. EMMPRIN ensures proper actomyosin-driven maturation of competent endothelial junctions by forming a molecular complex with gamma-catenin (also known as junction plakoglobin) and Nm23 (also known as NME1), a nucleoside diphosphate kinase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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