Full Product Name
TBX4 siRNA (Human)
Product Synonym Names
T-box transcription factor TBX4; T-box protein 4
Product Gene Name
TBX4 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P57082
Specificity
TBX4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TBX4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TBX4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TBX4 sirna
siRNA to inhibit TBX4 expression using RNA interference
Applications Tested/Suitable for TBX4 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TBX4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_060958.2
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NCBI GenBank Nucleotide #
NM_018488.2
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UniProt Primary Accession #
P57082
[Other Products]
UniProt Secondary Accession #
A5PKU7; B2RMT1; B7ZLV3[Other Products]
UniProt Related Accession #
P57082[Other Products]
Molecular Weight
60,275 Da
NCBI Official Full Name
T-box transcription factor TBX4
NCBI Official Synonym Full Names
T-box 4
NCBI Official Symbol
TBX4??[Similar Products]
NCBI Official Synonym Symbols
SPS
??[Similar Products]
NCBI Protein Information
T-box transcription factor TBX4
UniProt Protein Name
T-box transcription factor TBX4
Protein Family
T-box transcription factor
UniProt Gene Name
TBX4??[Similar Products]
UniProt Synonym Gene Names
T-box protein 4??[Similar Products]
UniProt Entry Name
TBX4_HUMAN
NCBI Summary for TBX4
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human homolog of mouse Tbx4, which is closely linked to Tbx2 on mouse chromosome 11. Similarly this gene, like TBX2, maps to human chromosome 17. Expression studies in mouse and chicken show that Tbx4 is expressed in developing hindlimb, but not in forelimb buds, suggesting a role for this gene in regulating limb development and specification of limb identity. [provided by RefSeq, Jul 2008]
UniProt Comments for TBX4
TBX4: Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Defects in TBX4 are the cause of small patella syndrome (SPS); also known as ischiopatellar dysplasia or Scott-Taor syndrome. SPS is an autosomal dominant skeletal dysplasia characterized by patellar aplasia or hypoplasia and by anomalies of the pelvis and feet, including disrupted ossification of the ischia and inferior pubic rami.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 17q21-q22
Cellular Component: nucleus
Molecular Function: DNA binding; transcription factor activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; skeletal morphogenesis; multicellular organismal development; morphogenesis of an epithelium; angiogenesis; limb morphogenesis; embryonic limb morphogenesis; lung development
Disease: Small Patella Syndrome
Research Articles on TBX4
1. a low level of TBX4 expression suggests a worse prognosis for patients with stage II PDAC. Down-regulation of the TBX4 gene in pancreas is less likely to be regulated by DNA methylation.
Precautions
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Disclaimer
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