For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat RPL10A. No significant cross-reactivity or interference between Rat RPL10A and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of RPL10A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283402 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 60S ribosomal protein L10a (RPL10A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RPL10A. The ELISA analytical biochemical technique of the MBS283402 kit is based on RPL10A antibody-RPL10A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPL10A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPL10A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RPL10A in samples. An antibody specific for RPL10A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRPL10A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RPL10A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RPL10A bound in the initial step. The color development is stopped and the intensity of the color is measured.

24,831 Da

ribosomal protein L10A

60S ribosomal protein L10a

60S ribosomal protein L10a

60S ribosomal subunit protein [RGD, Feb 2006]

RPL10A: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: focal adhesion; membrane; mitochondrion; nucleus

Molecular Function: RNA binding; structural constituent of ribosome

Biological Process: maturation of LSU-rRNA; response to ethanol; translation

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