14-3-3 protein sigma; stratifin; 14 3 3 protein sigma antibody; 14-3-3 protein sigma antibody; 1433S_HUMAN antibody; Epithelial cell marker protein 1 antibody; Er antibody; HME 1 antibody; HME1 antibody; MGC143283 antibody; Mkrn3 antibody; Mme1 antibody; OTTHUMP00000004242 antibody; RP23 137L22.11 antibody; SFN antibody; SFN protein antibody; Stratifin antibody; YWHAS antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-SFN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Rabbit IgG polyclonal antibody for 14-3-3 protein sigma(SFN) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)

Anti-14-3-3 sigma antibody, MBS175643, Western blotting
All lanes: Anti 14-3-3 sigma (MBS175643) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SE620 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD

Anti-14-3-3 sigma antibody, MBS175643, IHC(P)
IHC(P): Human Tonsil Tissue

Anti-14-3-3 sigma antibody, MBS175643, IHC(P)
IHC(P): Human Tonsil Tissue

Anti-14-3-3 sigma antibody, MBS175643, IHC(P)
IHC(P): Human Oesophagus Squama Cancer Tissue

24,336 Da

stratifin

14-3-3 protein sigma; 14-3-3 sigma; epithelial cell marker protein 1

14-3-3 protein sigma

HME1??[Similar Products]

1433S_HUMAN

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding

Biological Process: positive regulation of protein export from nucleus; regulation of epidermal cell division; release of cytochrome c from mitochondria; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; negative regulation of caspase activity; regulation of cyclin-dependent protein kinase activity; signal transduction; positive regulation of cell growth

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