Product Name
14-3-3 sigma, Polyclonal Antibody
Full Product Name
14-3-3 sigma Antibody
Product Synonym Names
1433S; 143S; Epithelial cell marker protein 1; HME1; SFN; Stratifin
Product Gene Name
anti-14-3-3 sigma antibody
[Similar Products]
Product Synonym Gene Name
SFN[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P31947
Species Reactivity
Human, Mouse
Specificity
14-3-3 sigma Antibody detects endogenous levels of total 14-3-3 sigma protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human SFN.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-14-3-3 sigma antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-14-3-3 sigma antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-14-3-3 sigma antibody
WB: 1:500~1:1000
ELISA: 1:1000
Western Blot (WB) of anti-14-3-3 sigma antibody
Western blot analysis of lysates from HT29 cells and RAW264.7 cells, using 14-3-3 sigma Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for 14-3-3 sigma. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006133.1
[Other Products]
NCBI GenBank Nucleotide #
NM_006142.4
[Other Products]
UniProt Primary Accession #
P31947
[Other Products]
UniProt Secondary Accession #
Q6FH30; Q6FH51; Q96DH0[Other Products]
UniProt Related Accession #
P31947[Other Products]
NCBI Official Full Name
14-3-3 protein sigma
NCBI Official Synonym Full Names
stratifin
NCBI Official Symbol
SFN??[Similar Products]
NCBI Official Synonym Symbols
YWHAS
??[Similar Products]
NCBI Protein Information
14-3-3 protein sigma
UniProt Protein Name
14-3-3 protein sigma
UniProt Synonym Protein Names
Epithelial cell marker protein 1; Stratifin
UniProt Gene Name
SFN??[Similar Products]
UniProt Synonym Gene Names
HME1??[Similar Products]
NCBI Summary for 14-3-3 sigma
This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]
UniProt Comments for 14-3-3 sigma
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
Research Articles on 14-3-3 sigma
1. 14-3-3sigma has a paracrine effect in educating stromal cells in tumor-associated microenvironment.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
