Product Name
14-3-3 sigma, Recombinant Protein
Popular Item
Full Product Name
14-3-3 sigma recombinant protein
Product Synonym Names
14-3-3 theta; YWHAQ; 1C5; HS1; 14-3-3; 14-3-3sigma Protein
Product Gene Name
14-3-3 sigma recombinant protein
[Similar Products]
Product Synonym Gene Name
14-3-3 sigma; SFN; stratifin; YWHAS[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (27189633..27190947). Location: 1p36.11
3D Structure
ModBase 3D Structure for P31947
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Type
Recombinant Fusion Protein
Source Note
Recombinant full-length human tag-free 14-3-3theta was expressed in E. coli cells.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Other Notes
Small volumes of 14-3-3 sigma recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
14-3-3 sigma recombinant protein
Recombinant full-length human tag-free 14-3-3sigma was expressed in E. coli.
Scientific Background: 14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.
Product Categories/Family for 14-3-3 sigma recombinant protein
Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins
Applications Tested/Suitable for 14-3-3 sigma recombinant protein
Western Blot (WB)
SDS-PAGE of 14-3-3 sigma recombinant protein
NCBI/Uniprot data below describe general gene information for 14-3-3 sigma. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006133.1
[Other Products]
NCBI GenBank Nucleotide #
NM_006142
[Other Products]
UniProt Primary Accession #
P31947
[Other Products]
UniProt Secondary Accession #
Q6FH30; Q6FH51; Q96DH0[Other Products]
UniProt Related Accession #
P31947[Other Products]
NCBI Official Full Name
Homo sapiens stratifin (SFN), mRNA
NCBI Official Synonym Full Names
stratifin
NCBI Official Symbol
SFN??[Similar Products]
NCBI Official Synonym Symbols
YWHAS
??[Similar Products]
NCBI Protein Information
14-3-3 protein sigma; 14-3-3 sigma; epithelial cell marker protein 1
UniProt Protein Name
14-3-3 protein sigma
UniProt Synonym Protein Names
Epithelial cell marker protein 1; Stratifin
UniProt Gene Name
SFN??[Similar Products]
UniProt Synonym Gene Names
HME1??[Similar Products]
UniProt Entry Name
1433S_HUMAN
UniProt Comments for 14-3-3 sigma
Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway
By similarity.p53-regulated inhibitor of G2/M progression.
Subunit structure: Homodimer. Interacts with KRT17 and SAMSN1
By similarity. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Interacts with COPS6. Interacts with RFWD2; this interaction leads to proteasomal degradation. Ref.8 Ref.10 Ref.13 Ref.16
Subcellular location: Cytoplasm. Nucleus
By similarity. Secreted. Note: May be secreted by a non-classical secretory pathway.
Tissue specificity: Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Sequence similarities: Belongs to the 14-3-3 family.
Product References and Citations for 14-3-3 sigma recombinant protein
1.Chan, T A. et al : 14-3-3-sigma is required to prevent mitotic catastrophe after DNA damage. Nature 401: 616-620, 1999.
2.Hermeking, H. et al: 14-3-3-sigma is a p53-regulated inhibitor of G2/M progression. Molec. Cell1: 3-11, 1997.
Research Articles on 14-3-3 sigma
1. 14-3-3 sigma is expressed in ovarian granulosa cell tumors and steroid cell tumors, but it is not expressed in ovarian fibromas, thecomas, Sertoli cell tumors, endometrial stromal sarcomas, and sex-cord stromal tumors, unclassified
Precautions
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Disclaimer
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