Product Name
PVRL1, siRNA
Full Product Name
PVRL1 siRNA (Mouse)
Product Synonym Names
HVEC; PRR1; Poliovirus receptor-related protein 1; Herpes virus entry mediator C; Herpesvirus entry mediator C; HveC; Nectin-1; CD antigen CD111
Product Gene Name
PVRL1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JKF6
Specificity
PVRL1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PVRL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PVRL1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PVRL1 sirna
siRNA to inhibit PVRL1 expression using RNA interference
Applications Tested/Suitable for PVRL1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PVRL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_067399.2
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NCBI GenBank Nucleotide #
NM_021424.2
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UniProt Primary Accession #
Q9JKF6
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UniProt Secondary Accession #
Q6P9M9; Q9ERL5; Q9JI17[Other Products]
UniProt Related Accession #
Q9JKF6[Other Products]
Molecular Weight
57,035 Da
NCBI Official Full Name
nectin-1
NCBI Official Synonym Full Names
poliovirus receptor-related 1
NCBI Official Symbol
Pvrl1??[Similar Products]
NCBI Official Synonym Symbols
PRR; HIgR; HveC; PRR1; Cd111; AI835281; AW549174; nectin-1
??[Similar Products]
NCBI Protein Information
nectin-1
UniProt Protein Name
Nectin-1
UniProt Synonym Protein Names
Herpes virus entry mediator C; Herpesvirus entry mediator C; HveC; Poliovirus receptor-related protein 1; CD_antigen: CD111
UniProt Gene Name
Pvrl1??[Similar Products]
UniProt Synonym Gene Names
Hvec; Prr1; Herpesvirus entry mediator C; HveC??[Similar Products]
UniProt Entry Name
PVRL1_MOUSE
UniProt Comments for PVRL1
nectin 1: Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4. Defects in PVRL1 are the cause of ectodermal dysplasia Margarita Island type (EDMI); also known as Zlotogora-Ogur syndrome, cleft lip/palate-ectodermal dysplasia syndrome (CLPED1) or ectodermal dysplasia 4. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDMI is an autosomal recessive syndrome characterized by the association of cleft lip/palate, ectodermal dysplasia (sparse short and dry scalp hair, sparse eyebrows and eyelashes), and partial syndactyly of the fingers and/or toes. Two thirds of the patients do not manifest oral cleft but present with abnormal teeth and nails. Defects in PVRL1 are the cause of non-syndromic orofacial cleft type 7 (OFC7). Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two- third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. Belongs to the nectin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell adhesion
Cellular Component: neuron projection; cell-cell adherens junction; intracellular membrane-bound organelle; membrane; axon; plasma membrane; integral to membrane; synapse; catenin complex; cell junction
Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; virion binding; receptor activity; cell adhesion molecule binding; carbohydrate binding
Biological Process: heterophilic cell adhesion; axon guidance; entry of virus into host cell; cell-cell adhesion; camera-type eye morphogenesis; viral reproduction; regulation of synaptogenesis; retina development in camera-type eye; lens morphogenesis in camera-type eye; homophilic cell adhesion; cell adhesion; iron ion transport
Research Articles on PVRL1
1. In summary, herpes simplex virus 1 entry into epidermis was shown to strongly depend on the presence of nectin-1, but the restricted presence of HVEM can potentially replace nectin-1 as a receptor.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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