Product Name
RPS6KA3, siRNA
Full Product Name
RPS6KA3 siRNA (Mouse)
Product Synonym Names
MAPKAPK1B; RPS6KA-RS1; RSK2; Ribosomal protein S6 kinase alpha-3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; p90RSK3; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; Ribosomal S6 kinase 2; RSK-2; pp90RSK2
Product Gene Name
RPS6KA3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P18654
Specificity
RPS6KA3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RPS6KA3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RPS6KA3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RPS6KA3 sirna
siRNA to inhibit RPS6KA3 expression using RNA interference
Applications Tested/Suitable for RPS6KA3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RPS6KA3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_683747.1
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NCBI GenBank Nucleotide #
NM_148945.2
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UniProt Primary Accession #
P18654
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UniProt Secondary Accession #
Q03140; Q8K3J8; B1AXN4[Other Products]
UniProt Related Accession #
P18654[Other Products]
Molecular Weight
83,694 Da
NCBI Official Full Name
ribosomal protein S6 kinase alpha-3
NCBI Official Synonym Full Names
ribosomal protein S6 kinase polypeptide 3
NCBI Official Symbol
Rps6ka3??[Similar Products]
NCBI Official Synonym Symbols
Rsk2; MPK-9; p90RSK3; pp90RSK2; MAPKAPK-1b; S6K-alpha3
??[Similar Products]
NCBI Protein Information
ribosomal protein S6 kinase alpha-3
UniProt Protein Name
Ribosomal protein S6 kinase alpha-3
UniProt Synonym Protein Names
90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; p90RSK3; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; Ribosomal S6 kinase 2; RSK-2; pp90RSK2
Protein Family
Ribosomal protein S6 kinase
UniProt Gene Name
Rps6ka3??[Similar Products]
UniProt Synonym Gene Names
Mapkapk1b; Rps6ka-rs1; Rsk2; S6K-alpha-3; p90-RSK 3; p90RSK3; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2??[Similar Products]
UniProt Entry Name
KS6A3_MOUSE
UniProt Comments for RPS6KA3
RSK2: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.
Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; RSK family; RSK subfamily
Cellular Component: cytoplasm; nucleus
Molecular Function: transferase activity; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups; caspase inhibitor activity; nucleotide binding; magnesium ion binding; kinase activity; ribosomal protein S6 kinase activity; protein kinase binding; ATP binding; protein kinase activity
Biological Process: regulation of translation; cellular response to stress; toll-like receptor signaling pathway; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; cell cycle; protein amino acid phosphorylation; phosphorylation
Research Articles on RPS6KA3
1. RSK2 regulates craniofacial development including tooth development and patterning via novel transcriptional targets
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
