For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NR2F2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7235150 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the COUP transcription factor 2 (NR2F2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NR2F2. The ELISA analytical biochemical technique of the MBS7235150 kit is based on NR2F2 antibody-NR2F2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NR2F2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NR2F2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Neurobiology

45,571 Da

nuclear receptor subfamily 2, group F, member 2

COUP transcription factor 2; COUP-TF II; COUP transcription factor II; apolipoprotein regulatory protein 1; apolipoprotein AI regulatory protein 1

COUP transcription factor 2

Aporp1; Arp-1; Arp1; Tfcoup2; COUP-TF2; ARP-1; COUP-TF II??[Similar Products]

COT2_MOUSE

NR2F2: Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. Interacts with SQSTM1. Binds DNA as a dimer; homodimer or heterodimer with NR2F6. Interacts with NCOA1, NCOA2, NCOA3 and PPARGC1A. Interacts with ZFPM2. Ubiquitous. Belongs to the nuclear hormone receptor family. NR2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; Motility/polarity/chemotaxis; DNA-binding

Cellular Component: nucleus

Molecular Function: ligand-dependent nuclear receptor activity; retinoic acid binding; protein homodimerization activity; DNA binding; zinc ion binding; sequence-specific DNA binding; metal ion binding; steroid hormone receptor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; limb development; skeletal muscle development; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; negative regulation of cyclin-dependent protein kinase activity; neuron migration; negative regulation of transcription from RNA polymerase II promoter; anterior/posterior pattern formation; regulation of transcription from RNA polymerase II promoter; fertilization; regulation of transcription, DNA-dependent; forebrain development; negative regulation of endothelial cell proliferation; blood vessel morphogenesis; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; radial pattern formation; maternal placenta development

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