Mouse Monoclonal [clone 4E10] (IgG1) to Human RPS6KA3 / RSK2; Human RPS6KA3 / RSK2; Coffin-Lowry syndrome; HU-2; ISPK1; MAPKAP kinase 1B; MAPKAPK-1b; MRX19; Ribosomal S6 kinase 2; RSK-2; p90-RSK 3; p90RSK3; RSK; S6K-alpha-3; S6K-alpha3; CLS; ISPK-1; MAPKAPK1B; p90-RSK2; Pp90RSK2

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

4E10

Mouse

Human RPS6KA3 / RSK2

Ascites

Ascites, 0.03% sodium azide

Long term: -20 degree C
Short term: 4 degree C
Avoid freeze-thaw cycles.

Small volumes of anti-RPS6KA3 / RSK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

RPS6KA3 Antibody, Coffin-Lowry syndrome Antibody, HU-2 Antibody, ISPK1 Antibody, MAPKAP kinase 1B Antibody, MAPKAPK-1b Antibody, MRX19 Antibody, Ribosomal S6 kinase 2 Antibody, RSK-2 Antibody, p90-RSK 3 Antibody, p90RSK3 Antibody, RSK Antibody, RSK2 Antibody, S6K-alpha-3 Antibody, S6K-alpha3 Antibody, CLS Antibody, ISPK-1 Antibody, MAPKAPK1B Antibody, p90-RSK2 Antibody, Pp90RSK2 Antibody Description: Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1.

Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)

ELISA (1:10000)
Flow Cytometry (1:200 - 1:400)
ICC (1:200 - 1:1000)
IF (1:1000)
IHC-P (1:50)
WB (1:500 - 1:2000)

Anti-RPS6KA3 / RSK2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

Immunofluorescence of HepG2 cells using RSK2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot using RSK2 monoclonal antibody against human RSK2 (AA: 1-212) recombinant protein. (Expected MW is 49.7 kDa)

Western blot using RSK2 mouse monoclonal antibody against HeLa (1), MCF-7 (2), and HepG2 (3) cell lysate.

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

83,736 Da

ribosomal protein S6 kinase, 90kDa, polypeptide 3

ribosomal protein S6 kinase alpha-3

Ribosomal protein S6 kinase alpha-3

ISPK1; MAPKAPK1B; RSK2; S6K-alpha-3; p90-RSK 3; p90RSK3; ISPK-1; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2??[Similar Products]

KS6A3_HUMAN

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]

RSK2: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: Xp22.2-p22.1

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: ATP binding; caspase inhibitor activity; magnesium ion binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: axon guidance; cell cycle; central nervous system development; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of caspase activity; nerve growth factor receptor signaling pathway; positive regulation of cell differentiation; positive regulation of cell growth; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription in response to stress; regulation of translation in response to stress; response to lipopolysaccharide; signal transduction; skeletal development; stress-activated MAPK cascade; synaptic transmission; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway

Disease: Coffin-lowry Syndrome; Mental Retardation, X-linked 19

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