Anti -RSK1, RSK2, phosphorylated (Ser221/Ser227) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ri

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: X; NC_000086.7 (159256120..159363485). Location: X F4; X 73.27 cM

Polyclonal

Rabbit

Recognizes human Rsk1, Rsk2, phosphorylated, Ser221, Ser227.. Species Crossreactivity

Affinity Purified
Purified by affinity chromatography.

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-Rps6ka3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c) and RSK4. RSK proteins are substrates for and downstream transducers of, MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization and modulation of complex formation with ERK1&2 and/or 14-3-3beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock and irradiation. ERK1&2 phosphorylate threonine 573 (threonine 577 in RSK2) of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serines 363 and 380 (serines 369 and 386 in RSK2) within the linker region. This phosphorylation is required for activation of the N-terminal kinase domain by PDK1. The activated N-terminal kinase domain phosphorylates various substrates that include transporters, transcription factors and transcription co-activators.

Antibodies; Abs to Protein Kinases, Ribosomal

Western Blot (WB)

Suitable for use in Western Blot. Other applications not tested
Dilution: Western Blot: 1:1000
Optimal dilution determined by the researcher.

83,694 Da[Similar Products]

ribosomal protein S6 kinase polypeptide 3

ribosomal protein S6 kinase alpha-3; RSK-2; p90RSK3; p90-RSK 3; MAPKAPK-1b; S6K-alpha-3; MAPKAP kinase 1b; ribosomal S6 kinase 2; ribosomal protein S6 kinase 2; MAPK-activated protein kinase 1b; 90 kDa ribosomal protein S6 kinase 3; MAP kinase-activated protein kinase 1b

Ribosomal protein S6 kinase alpha-3

Mapkapk1b; Rps6ka-rs1; Rsk2; S6K-alpha-3; p90-RSK 3; p90RSK3; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2??[Similar Products]

KS6A3_MOUSE

RSK2: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; RSK family; RSK subfamily

Cellular Component: cytoplasm; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; caspase inhibitor activity; transferase activity, transferring phosphorus-containing groups; magnesium ion binding; nucleotide binding; kinase activity; ribosomal protein S6 kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: regulation of translation; cellular response to stress; toll-like receptor signaling pathway; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; cell cycle; phosphorylation; protein amino acid phosphorylation

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