Product Name
Endonuclease 8-like 1 (Neil1), Recombinant Protein
Full Product Name
Recombinant Mouse Endonuclease 8-like 1 (Neil1)
Product Gene Name
Neil1 recombinant protein
[Similar Products]
Product Synonym Gene Name
Neil1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-389aa, Full Length of Mature Protein
Sequence
PEGPELHLAS HFVNETCKGL VFGGCVEKSS VSRNPEVPFE SSAYHISALA RGKELRLTLS PLPGSQPPQK PLSLVFRFGM SGSFQLVPAE ALPRHAHLRF YTAPPAPRLA LCFVDIRRFG HWDPGGEWQP GRGPCVLLEY ERFRENVLRN LSDKAFDRPI CEALLDQRFF NGIGNYLRAE ILYRLKIPPF EKARTVLEAL QQCRPSPELT LSQKIKAKLQ NPDLLELCHL VPKEVVQLGG KGYGPERGEE DFAAFRAWLR CYGVPGMSSL RDRHGRTIWF QGDPGPLAPK GGRSQKKKSQ ETQLGAEDRK EDLPLSSKSV SRMRRARKHP PKRIAQQSEG AGLQQNQETP TAPEKGKRRG QRASTGHRRR PKTIPDTRPR EAGESSAS
3D Structure
ModBase 3D Structure for Q8K4Q6
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Neil1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for Neil1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_082623.1
[Other Products]
NCBI GenBank Nucleotide #
NM_028347.3
[Other Products]
UniProt Primary Accession #
Q8K4Q6
[Other Products]
UniProt Secondary Accession #
Q80V58; Q9CYT9[Other Products]
UniProt Related Accession #
Q8K4Q6[Other Products]
Molecular Weight
28,453 Da
NCBI Official Full Name
endonuclease 8-like 1 isoform 1
NCBI Official Synonym Full Names
nei endonuclease VIII-like 1 (E. coli)
NCBI Official Symbol
Neil1??[Similar Products]
NCBI Official Synonym Symbols
NEH1; Nei1; 2810450N13Rik
??[Similar Products]
NCBI Protein Information
endonuclease 8-like 1
UniProt Protein Name
Endonuclease 8-like 1
UniProt Synonym Protein Names
DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; Nei homolog 1; NEH1; Nei-like protein 1
Protein Family
Endonuclease
UniProt Gene Name
Neil1??[Similar Products]
UniProt Synonym Gene Names
Nei1; NEH1??[Similar Products]
UniProt Comments for Neil1
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.
Research Articles on Neil1
1. Collectively the data suggest that NEIL1-initiated repair of a subset of ROS-induced DNA base lesions may be insufficient to prevent the initiation of inflammatory pathways during chronic UV exposure in mouse skin.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
