Human TFIIH basal transcription factor complex helicase XPD subunit (ERCC2) ELISA kit; COFS2; EM9; MGC102762; MGC126218; MGC126219; TTD; XPD; excision repair cross-complementing rodent repair deficiency; complementation group 2 protein; xeroderma pigmentosum complementary group D; excision repair cross-complementing rodent repair deficiency; complementation group 2

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of ERCC2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9336264 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the excision repair cross-complementing rodent repair deficiency, complementation group 2 (ERCC2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ERCC2. The ELISA analytical biochemical technique of the MBS9336264 kit is based on ERCC2 antibody-ERCC2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ERCC2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ERCC2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

86,909 Da

excision repair cross-complementation group 2

TFIIH basal transcription factor complex helicase XPD subunit; CXPD; BTF2 p80; TFIIH p80; TFIIH 80 kDa subunit; DNA excision repair protein ERCC-2; DNA repair protein complementing XP-D cells; basic transcription factor 2 80 kDa subunit; xeroderma pigmentosum complementary group D; xeroderma pigmentosum group D-complementing protein; TFIIH basal transcription factor complex 80 kDa subunit; TFIIH basal transcription factor complex helicase subunit; TFIIH basal transcription factor complex helicase XPB subunit; excision repair cross-complementing rodent repair deficiency, complementation group 2

TFIIH basal transcription factor complex helicase XPD subunit

XPD; XPDC; BTF2 p80; TFIIH 80 kDa subunit; TFIIH p80??[Similar Products]

ERCC2_HUMAN

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

ERCC2: ATP-dependent 5'-3' DNA helicase, component of the core- TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. Might also have a role in aging process and could play a causative role in the generation of skin cancers. Belongs to the helicase family. RAD3/XPD subfamily. One of the six subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. The interaction with GTF2H2 results in the stimulation of the 5'-->3' helicase activity. Interacts with Epstein-Barr virus EBNA2.

Protein type: Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; holo TFIIH complex; cytoplasm; spindle; nucleus; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: RNA polymerase subunit kinase activity; ATP-dependent DNA helicase activity; protein C-terminus binding; DNA-dependent ATPase activity; protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; protein N-terminus binding; 5'-3' DNA helicase activity; ATP binding; protein kinase activity

Biological Process: transcription from RNA polymerase II promoter; extracellular matrix organization and biogenesis; erythrocyte maturation; viral reproduction; positive regulation of viral transcription; apoptosis; positive regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic; protein amino acid phosphorylation; bone mineralization; embryonic cleavage; post-embryonic development; chromosome segregation; mRNA capping; UV protection; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; positive regulation of DNA binding; aging; transcription initiation from RNA polymerase II promoter; in utero embryonic development; multicellular organism growth; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; hair cell differentiation; nucleotide-excision repair, DNA incision; DNA repair; termination of RNA polymerase I transcription; hair follicle maturation; DNA duplex unwinding; cell proliferation; myelin formation in the central nervous system; nucleotide-excision repair; spinal cord development; RNA elongation from RNA polymerase II promoter; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; transcription initiation from RNA polymerase I promoter

Disease: Cerebrooculofacioskeletal Syndrome 2; Xeroderma Pigmentosum, Complementation Group D; Trichothiodystrophy, Photosensitive

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