Full Product Name
POLH siRNA (Mouse)
Product Synonym Names
RAD30A; XPV; DNA polymerase eta; RAD30 homolog A; Xeroderma pigmentosum variant type protein homolog
Product Gene Name
POLH sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JJN0
Specificity
POLH siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse POLH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of POLH sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
POLH sirna
siRNA to inhibit POLH expression using RNA interference
Applications Tested/Suitable for POLH sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for POLH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_109640.1
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NCBI GenBank Nucleotide #
NM_030715.3
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UniProt Primary Accession #
Q9JJN0
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UniProt Secondary Accession #
Q9JJJ2[Other Products]
UniProt Related Accession #
Q9JJN0[Other Products]
Molecular Weight
76,167 Da
NCBI Official Full Name
DNA polymerase eta
NCBI Official Synonym Full Names
polymerase (DNA directed), eta (RAD 30 related)
NCBI Official Symbol
Polh??[Similar Products]
NCBI Official Synonym Symbols
XPV; RAD30A
??[Similar Products]
NCBI Protein Information
DNA polymerase eta
UniProt Protein Name
DNA polymerase eta
UniProt Synonym Protein Names
RAD30 homolog A; Xeroderma pigmentosum variant type protein homolog
Protein Family
DNA polymerase
UniProt Gene Name
Polh??[Similar Products]
UniProt Synonym Gene Names
Rad30a; Xpv??[Similar Products]
UniProt Entry Name
POLH_MOUSE
UniProt Comments for POLH
POLH: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. Interacts with REV1. Interacts with monoubiquitinated PCNA, but not unmodified PCNA. Interacts with POLI. Belongs to the DNA polymerase type-Y family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transferase; EC 2.7.7.7; DNA repair, damage
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: transferase activity; DNA binding; metal ion binding; DNA-directed DNA polymerase activity; damaged DNA binding; nucleotidyltransferase activity
Biological Process: DNA synthesis during DNA repair; postreplication repair; pyrimidine dimer repair; DNA replication; DNA repair; response to DNA damage stimulus; response to UV-C
Research Articles on POLH
1. POLH deficiency did not affect the frequency and patterns of C:G mutations and UNG POLH double deficiency showed an additive effect of single deficiency.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
