Full Product Name
POLH siRNA (Human)
Product Synonym Names
RAD30; RAD30A; XPV; DNA polymerase eta; RAD30 homolog A; Xeroderma pigmentosum variant type protein
Product Gene Name
POLH sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y253
Specificity
POLH siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POLH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of POLH sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
POLH sirna
siRNA to inhibit POLH expression using RNA interference
Applications Tested/Suitable for POLH sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for POLH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001278899.1
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NCBI GenBank Nucleotide #
NM_001291970.1
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UniProt Primary Accession #
Q9Y253
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UniProt Secondary Accession #
Q7L8E3; Q96BC4; Q9BX13[Other Products]
UniProt Related Accession #
Q9Y253[Other Products]
Molecular Weight
46,283 Da
NCBI Official Full Name
DNA polymerase eta isoform 3
NCBI Official Synonym Full Names
polymerase (DNA directed), eta
NCBI Official Symbol
POLH??[Similar Products]
NCBI Official Synonym Symbols
XPV; XP-V; RAD30; RAD30A
??[Similar Products]
NCBI Protein Information
DNA polymerase eta
UniProt Protein Name
DNA polymerase eta
UniProt Synonym Protein Names
RAD30 homolog A; Xeroderma pigmentosum variant type protein
Protein Family
DNA polymerase
UniProt Gene Name
POLH??[Similar Products]
UniProt Synonym Gene Names
RAD30; RAD30A; XPV??[Similar Products]
UniProt Entry Name
POLH_HUMAN
NCBI Summary for POLH
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Comments for POLH
POLH: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. Interacts with REV1. Interacts with monoubiquitinated PCNA, but not unmodified PCNA. Interacts with POLI. Belongs to the DNA polymerase type-Y family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; EC 2.7.7.7; Transferase
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein binding; metal ion binding; DNA-directed DNA polymerase activity; damaged DNA binding
Biological Process: regulation of DNA repair; DNA synthesis during DNA repair; postreplication repair; pyrimidine dimer repair; DNA repair; DNA replication; response to UV-C
Disease: Xeroderma Pigmentosum, Variant Type
Research Articles on POLH
1. The results show that the FF483-484 amino acids in the human Poleta (designated F1 motif) are necessary for the interaction of this polymerase with POLD2, the B subunit of the replicative DNA polymerase delta, both in vitro and in vivo.
Precautions
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Disclaimer
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