Full Product Name
POLH Antibody, FITC conjugated
Product Synonym Names
DNA polymerase eta; RAD30 homolog A; Xeroderma pigmentosum variant type protein; POLH; RAD30; RAD30A; XPV
Product Gene Name
anti-POLH antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y253
Purity/Purification
>95%, Protein G purified
Immunogen
Recombinant Human DNA polymerase eta protein (408-609AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-POLH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-POLH antibody
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.
Applications Tested/Suitable for anti-POLH antibody
ELISA (EIA)
NCBI/Uniprot data below describe general gene information for POLH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001278899.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001291970.1
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UniProt Primary Accession #
Q9Y253
[Other Products]
UniProt Secondary Accession #
Q7L8E3; Q96BC4; Q9BX13[Other Products]
UniProt Related Accession #
Q9Y253[Other Products]
Molecular Weight
46,283 Da
NCBI Official Full Name
DNA polymerase eta isoform 3
NCBI Official Synonym Full Names
DNA polymerase eta
NCBI Official Symbol
POLH??[Similar Products]
NCBI Official Synonym Symbols
XPV; XP-V; RAD30; RAD30A
??[Similar Products]
NCBI Protein Information
DNA polymerase eta
UniProt Protein Name
DNA polymerase eta
UniProt Synonym Protein Names
RAD30 homolog A; Xeroderma pigmentosum variant type protein
Protein Family
DNA polymerase
UniProt Gene Name
POLH??[Similar Products]
UniProt Synonym Gene Names
RAD30; RAD30A; XPV??[Similar Products]
NCBI Summary for POLH
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Comments for POLH
POLH: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci. Interacts with REV1. Interacts with monoubiquitinated PCNA, but not unmodified PCNA. Interacts with POLI. Belongs to the DNA polymerase type-Y family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; EC 2.7.7.7; Transferase
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: cytoplasm; nucleoplasm
Molecular Function: DNA-directed DNA polymerase activity; protein binding
Biological Process: bypass DNA synthesis; DNA repair; DNA synthesis during DNA repair; regulation of DNA repair; response to UV-C
Disease: Xeroderma Pigmentosum, Variant Type
Research Articles on POLH
1. These results provide important knowledge about the effects of the length and structure of the alkyl group in O(4)-alkylthymidine lesions on the fidelity and efficiency of DNA replication mediated by human DNA polymerase eta.
Precautions
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Disclaimer
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