Full Product Name
POLH Antibody
Product Gene Name
anti-POLH antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human
Predicted: Mouse, Rat
Form/Format
Purified by protein affinity column containing 1% BSA and 0.02% NaN3.
Preparation and Storage
Already reconstituted in 200ul sterile H2O. The antibody is stable for at least one year when stored at-20 degree C after reconstitution. Avoid repeated freeze/defrost cycles.
Other Notes
Small volumes of anti-POLH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-POLH antibody
Rabbit polyclonal POLH (1) antibody was raised against a recombinated human POLH protein 76-277aa (BC015742).
Product Categories/Family for anti-POLH antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-POLH antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-POLH antibody
ELISA: 1:20,000-1:80,000
WB: 1:1,000-1:2,000
Testing Data of anti-POLH antibody
NCBI/Uniprot data below describe general gene information for POLH. It may not necessarily be applicable to this product.
NCBI Accession #
BC015742
[Other Products]
UniProt Secondary Accession #
Q7L8E3; Q96BC4; Q9BX13[Other Products]
UniProt Related Accession #
Q9Y253[Other Products]
Molecular Weight
46,283 Da
NCBI Official Full Name
Homo sapiens polymerase (DNA directed), eta, mRNA
NCBI Official Synonym Full Names
DNA polymerase eta
NCBI Official Symbol
POLH??[Similar Products]
NCBI Official Synonym Symbols
XPV; XP-V; RAD30; RAD30A
??[Similar Products]
NCBI Protein Information
DNA polymerase eta
UniProt Protein Name
DNA polymerase eta
UniProt Synonym Protein Names
RAD30 homolog A; Xeroderma pigmentosum variant type protein
Protein Family
DNA polymerase
UniProt Gene Name
POLH??[Similar Products]
UniProt Synonym Gene Names
RAD30; RAD30A; XPV??[Similar Products]
NCBI Summary for POLH
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Comments for POLH
DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940). Targets POLI to replication foci (PubMed:12606586).
Product References and Citations for anti-POLH antibody
(1) Pustovalova, Y., Bezsonova, I., Korzhnev, D.M. The C-terminal domain of human Rev1 contains independent s for DNA polymerase eta and Rev7 subunit of polymerase zeta. FEBS Lett. 586 (19), 3051-3056 (2012).
Research Articles on POLH
1. POLH & POLK are both able to exchange with PolD1 stalled at repetitive CFS (common fragile sites) sequences. POLD1 synthesis was inhibited by replication stress caused by aphidicolin, preventing any replication past CFS. Importantly, POLH & POLK were still proficient in rescuing this stalled POLD1 synthesis. POLD1 stalling at CFSs allows for free exchange with specialized polymerase that is not driven by PCNA.
Precautions
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