Full Product Name
HSF4 siRNA (Human)
Product Synonym Names
Heat shock factor protein 4; HSF 4; hHSF4; Heat shock transcription factor 4; HSTF 4
Product Gene Name
HSF4 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9ULV5
Specificity
HSF4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HSF4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HSF4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HSF4 sirna
siRNA to inhibit HSF4 expression using RNA interference
Applications Tested/Suitable for HSF4 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HSF4. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001035757.1
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NCBI GenBank Nucleotide #
NM_001040667.2
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UniProt Primary Accession #
Q9ULV5
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UniProt Secondary Accession #
Q99472; Q9ULV6[Other Products]
UniProt Related Accession #
Q9ULV5[Other Products]
Molecular Weight
49,952 Da
NCBI Official Full Name
heat shock factor protein 4 isoform b
NCBI Official Synonym Full Names
heat shock transcription factor 4
NCBI Official Symbol
HSF4??[Similar Products]
NCBI Official Synonym Symbols
CTM; CTRCT5
??[Similar Products]
NCBI Protein Information
heat shock factor protein 4
UniProt Protein Name
Heat shock factor protein 4
UniProt Synonym Protein Names
Heat shock transcription factor 4; HSTF 4
Protein Family
Heat shock factor protein
UniProt Gene Name
HSF4??[Similar Products]
UniProt Synonym Gene Names
HSF 4; hHSF4; HSTF 4??[Similar Products]
UniProt Entry Name
HSF4_HUMAN
NCBI Summary for HSF4
Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for HSF4
HSF4: DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription. Defects in HSF4 are the cause of cataract, zonular (CZ). A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes. Defects in HSF4 are the cause of cataract Marner type (CAM). A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription, coactivator/corepressor; Cell development/differentiation
Chromosomal Location of Human Ortholog: 16q21
Cellular Component: nucleoplasm
Molecular Function: sequence-specific DNA binding; transcription factor activity; protein phosphatase binding; transcription corepressor activity
Biological Process: histone H3-K9 demethylation; camera-type eye development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; cell development
Disease: Cataract 5, Multiple Types
Research Articles on HSF4
1. T in HSF4 DNA may be responsible for the autosomal dominant congenital cataract in this family">concluded that the new mutation of c.331C>T in HSF4 DNA may be responsible for the autosomal dominant congenital cataract in this family
Precautions
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Disclaimer
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