For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of GP- II elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS745432 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycogen phosphorylase II (GP- II) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GP- II. The ELISA analytical biochemical technique of the MBS745432 kit is based on GP- II antibody-GP- II antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GP- II antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GP- II. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This GP-II ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GP-II. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: GP-II ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GP-II antibody and an GP-II-HRP conjugate. The assay sample and buffer are incubated together with GP-II-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GP-II concentration since GP- II from samples and GP-II-HRP conjugate compete for the anti-GP-II antibody . Since the number of sites is limited, as more sites are occupied by GP-II from the sample, fewer sites are left to bind GP-II-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GP-II concentration in each sample is interpolated from this standard curve.

93,134 Da

phosphorylase, glycogen, liver

glycogen phosphorylase, liver form

Glycogen phosphorylase, liver form

PYGL_HUMAN

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011]

PYGL: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected. Belongs to the glycogen phosphorylase family.

Protein type: Transferase; EC 2.4.1.1; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 14q21-q22

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: glycogen phosphorylase activity; protein homodimerization activity; bile acid binding; purine binding; drug binding; vitamin binding; ATP binding; glucose binding; pyridoxal phosphate binding; AMP binding

Biological Process: glycogen metabolic process; glycogen catabolic process; carbohydrate metabolic process; 5-phosphoribose 1-diphosphate biosynthetic process; glucose metabolic process; pathogenesis; glucose homeostasis

Disease: Glycogen Storage Disease Vi

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