Full Product Name
LRP5 siRNA (Human)
Product Synonym Names
LR3; LRP7; Low-density lipoprotein receptor-related protein 5; LRP-5
Product Gene Name
LRP5 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O75197
Specificity
LRP5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LRP5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of LRP5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LRP5 sirna
siRNA to inhibit LRP5 expression using RNA interference
Applications Tested/Suitable for LRP5 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for LRP5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001278831.1
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NCBI GenBank Nucleotide #
NM_001291902.1
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UniProt Primary Accession #
O75197
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UniProt Secondary Accession #
Q96TD6; Q9UES7; Q9UP66[Other Products]
UniProt Related Accession #
O75197[Other Products]
Molecular Weight
179,145 Da
NCBI Official Full Name
low-density lipoprotein receptor-related protein 5 isoform 2
NCBI Official Synonym Full Names
low density lipoprotein receptor-related protein 5
NCBI Official Symbol
LRP5??[Similar Products]
NCBI Official Synonym Symbols
HBM; LR3; OPS; EVR1; EVR4; LRP7; OPPG; BMND1; LRP-5; OPTA1; VBCH2
??[Similar Products]
NCBI Protein Information
low-density lipoprotein receptor-related protein 5
UniProt Protein Name
Low-density lipoprotein receptor-related protein 5
Protein Family
Low-density lipoprotein receptor-related protein
UniProt Gene Name
LRP5??[Similar Products]
UniProt Synonym Gene Names
LR3; LRP7; LRP-5??[Similar Products]
UniProt Entry Name
LRP5_HUMAN
NCBI Summary for LRP5
This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Comments for LRP5
LRP5: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation. Homodimer; disulfide-linked. Forms phosphorylated oligomer aggregates on Wnt-signaling. Component of a Wnt-signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts with FZD8; the interaction is formed on WNT-binding and signaling. Interacts (via the phosphorylated PPPSP motif domains) with AXIN1; the interaction prevents inhibition of beta-catenin phosphorylation and signaling and is enhanced in the presence of GSK3B and WNT1 or WNT3A. Interacts (via beta-propeller regions 3 and 4) with DKK1; the interaction, enhanced by MESD and/or KREMEN, inhibits beta-catenin signaling by preventing GSK3-mediated phosphorylation of the PPPSP motifs and subsequent, AXIN1 binding. Interacts with MESD; the interaction prevents the formation of LRP5 aggregates, targets LRP5 to the plasma membrane and, when complexed with KREMEN2, increases DKK1 binding. Interacts with CSNK1E. Interacts with SOST; the interaction antagonizes canonical Wnt signaling. Interacts with APCDD1. Widely expressed, with the highest level of expression in the liver and in aorta. Belongs to the LDLR family.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13.4
Cellular Component: mitochondrion; endoplasmic reticulum; integral to membrane; plasma membrane; receptor complex
Molecular Function: Wnt-protein binding; Wnt receptor activity; protein binding; toxin transporter activity; coreceptor activity
Biological Process: response to peptide hormone stimulus; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; bone remodeling; anatomical structure regression; regulation of apoptosis; anterior/posterior pattern formation; regulation of blood pressure; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; cell migration involved in gastrulation; embryonic limb morphogenesis; cholesterol metabolic process; Wnt receptor signaling pathway; positive regulation of mitosis; regulation of bone remodeling; endocytosis; gastrulation with mouth forming second; osteoblast development; bone marrow development; glucose catabolic process; cholesterol homeostasis; embryonic retina morphogenesis in camera-type eye; positive regulation of osteoblast proliferation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; retina morphogenesis in camera-type eye
Disease: Osteoporosis-pseudoglioma Syndrome; Van Buchem Disease, Type 2; Exudative Vitreoretinopathy 1; Osteopetrosis, Autosomal Dominant 1; Exudative Vitreoretinopathy 4; Bone Mineral Density Quantitative Trait Locus 1; Osteoporosis; Endosteal Hyperostosis, Autosomal Dominant
Research Articles on LRP5
1. mechanism of LRP protein action in the process of bone tissue metabolism and etiology of osteoporosis.
Precautions
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