Antigen-Affinity Purified Polyclonal Antibodies Anti-human APRIL

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 17; NC_000017.11 (7558292..7561608). Location: 17p13.1

Polyclonal

Rabbit

Affinity chromatography

Lyophilized

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-APRIL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the Nor C-terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.

ELISA (EIA), Western Blot (WB)

Western Blot: To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5 - 3.0 ng/lane, under either reducing or nonreducing conditions.

27,433 Da

tumor necrosis factor (ligand) superfamily, member 13

tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2

Tumor necrosis factor ligand superfamily member 13

APRIL; TALL2; ZTNF2; APRIL; TALL-2; TRDL-1??[Similar Products]

TNF13_HUMAN

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Function: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Ref.12

Subunit structure: Homotrimer

Potential.

Subcellular location: Secreted Ref.13.

Tissue specificity: Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.

Induction: Down-regulated by phorbol myristate acetate/ionomycin treatment.

Post-translational modification: The precursor is cleaved by furin. Ref.13

Sequence similarities: Belongs to the tumor necrosis factor family.

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