For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of BCL6. No significant cross-reactivity or interference between BCL6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BCL6 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of BCL6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7203268 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the B cell lymphoma 6 protein (BCL6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BCL6. The ELISA analytical biochemical technique of the MBS7203268 kit is based on BCL6 antibody-BCL6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BCL6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BCL6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: BCL6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BCL6 antibody and an BCL6-HRP conjugate. The assay sample and buffer are incubated together with BCL6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL6 concentration since BCL6 from samples and BCL6-HRP conjugate compete for the anti-BCL6 antibody . Since the number of sites is limited, as more sites are occupied by BCL6 from the sample, fewer sites are left to bind BCL6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL6 concentration in each sample is interpolated from this standard curve.

Epigenetics and Nuclear Signaling

72,367 Da

B-cell CLL/lymphoma 6

B-cell lymphoma 6 protein; BCL-5; BCL-6; protein LAZ-3; zinc finger protein 51; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein transcript; zinc finger transcription factor BCL6S; cys-his2 zinc finger transcription factor; zinc finger and BTB domain-containing protein 27; lymphoma-associated zinc finger gene on chromosome 3

B-cell lymphoma 6 protein

BCL5; LAZ3; ZBTB27; ZNF51; BCL-6; BCL-5??[Similar Products]

BCL6_HUMAN

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Bcl-6: a zinc finger transcription factor and contains an N-terminal BTB/POZ domain. A sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. Mediates transcriptional repression and interacts with components of histone deacetylase co-repressor complexes including N-CoR and SMRT. The gene is frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL.

Protein type: Oncoprotein; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: replication fork; nucleus

Molecular Function: protein binding; chromatin DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: erythrocyte development; positive regulation of apoptosis; regulation of memory T cell differentiation; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; Rho protein signal transduction; negative regulation of cell proliferation; negative regulation of isotype switching to IgE isotypes; positive regulation of histone deacetylation; positive regulation of B cell proliferation; germinal center formation; negative regulation of mast cell cytokine production; inflammatory response; negative regulation of T-helper 2 cell differentiation; regulation of immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of cell motility; negative regulation of cell-matrix adhesion; regulation of germinal center formation; negative regulation of Rho protein signal transduction; protein import into nucleus, translocation; B cell differentiation; regulation of inflammatory response; negative regulation of Notch signaling pathway; spermatogenesis; negative regulation of cell growth; actin cytoskeleton organization and biogenesis; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation; response to DNA damage stimulus; negative regulation of B cell apoptosis

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