Product Name
Homogentisate-1,2-Dioxygenase (HGD), Recombinant Protein
Full Product Name
Recombinant Homogentisate-1,2-Dioxygenase (HGD)
Product Synonym Names
AKU; HGO; Homogentisic Acid Oxidase; Homogentisicase; Homogentisate oxygenase
Product Gene Name
HGD recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
Ser8~Glu428
3D Structure
ModBase 3D Structure for Q93099
Host
Host: E Coli
Source: Prokaryotic Expression
Purity/Purification
> 90%
Form/Format
Freeze-Dried Powder; PBS, pH7.4, Containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Tag
N-terminal His and GST-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Subcellular Location
Cytosol, Secreted
Reconstitution
Reconstitute in PBS or others.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of HGD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for HGD recombinant protein
Enzyme & Kinase
Applications Tested/Suitable for HGD recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
NCBI/Uniprot data below describe general gene information for HGD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000178.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000187.4
[Other Products]
UniProt Primary Accession #
Q93099
[Other Products]
UniProt Related Accession #
Q93099[Other Products]
Molecular Weight
Predicted MW: 77.2kDa
Accurate MW: 77kDa
NCBI Official Full Name
homogentisate 1,2-dioxygenase
NCBI Official Synonym Full Names
homogentisate 1,2-dioxygenase
NCBI Official Symbol
HGD??[Similar Products]
NCBI Official Synonym Symbols
AKU; HGO
??[Similar Products]
NCBI Protein Information
homogentisate 1,2-dioxygenase
UniProt Protein Name
Homogentisate 1,2-dioxygenase
UniProt Synonym Protein Names
Homogentisate oxygenase; Homogentisic acid oxidase; Homogentisicase
Protein Family
2-(hydroxymethyl)glutarate dehydrogenase
UniProt Gene Name
HGD??[Similar Products]
UniProt Synonym Gene Names
HGO??[Similar Products]
UniProt Entry Name
HGD_HUMAN
NCBI Summary for HGD
This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010]
Research Articles on HGD
1. Twelve novel HGD gene variants have been identified in 99 alkaptonuria patients affecting the bones.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.