Product Name
ATP11A, siRNA
Full Product Name
ATP11A siRNA (Human)
Product Synonym Names
ATPIH; ATPIS; KIAA1021; Probable phospholipid-transporting ATPase IH; ATPase IS; ATPase class VI type 11A
Product Gene Name
ATP11A sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P98196
Specificity
ATP11A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATP11A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ATP11A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ATP11A sirna
siRNA to inhibit ATP11A expression using RNA interference
Applications Tested/Suitable for ATP11A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ATP11A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_056020.2
[Other Products]
NCBI GenBank Nucleotide #
NM_015205.2
[Other Products]
UniProt Primary Accession #
P98196
[Other Products]
UniProt Secondary Accession #
Q5VXT2[Other Products]
UniProt Related Accession #
P98196[Other Products]
Molecular Weight
129,756 Da
NCBI Official Full Name
probable phospholipid-transporting ATPase IH isoform a
NCBI Official Synonym Full Names
ATPase, class VI, type 11A
NCBI Official Symbol
ATP11A??[Similar Products]
NCBI Official Synonym Symbols
ATPIH; ATPIS
??[Similar Products]
NCBI Protein Information
probable phospholipid-transporting ATPase IH
UniProt Protein Name
Probable phospholipid-transporting ATPase IH
UniProt Synonym Protein Names
ATPase IS; ATPase class VI type 11A; P4-ATPase flippase complex alpha subunit ATP11A
Protein Family
Probable phospholipid-transporting ATPase
UniProt Gene Name
ATP11A??[Similar Products]
UniProt Synonym Gene Names
ATPIH; ATPIS; KIAA1021??[Similar Products]
UniProt Entry Name
AT11A_HUMAN
NCBI Summary for ATP11A
The protein encoded by this gene is an integral membrane ATPase. The encoded protein is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium across membranes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ATP11A
ATP11A: is an integral membrane ATPase. The encoded protein is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium across membranes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Protein type: Hydrolase; Transporter; Membrane protein, integral; EC 3.6.3.1; Membrane protein, multi-pass; Transporter, ion channel
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: recycling endosome; membrane; intracellular membrane-bound organelle; lysosomal membrane; endoplasmic reticulum; early endosome; plasma membrane; integral to membrane
Molecular Function: phospholipid-translocating ATPase activity; protein binding; magnesium ion binding; ATP binding
Biological Process: phospholipid translocation; metabolic process
Research Articles on ATP11A
1. Observational study, meta-analysis, and genome-wide association study of gene-disease association. (HuGE Navigator)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
