Product Name
CST complex subunit STN1 (Obfc1), Recombinant Protein
Full Product Name
Recombinant Mouse CST complex subunit STN1 (Obfc1)
Product Gene Name
Obfc1 recombinant protein
[Similar Products]
Product Synonym Gene Name
Obfc1[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-378aa, Full Length of Mature Protein
Sequence
PQPCLLMECE SSPREEEIPP LFWGLDPVFL AFAKLYIKDI LEMKESQQVP GTYFYNGHPI RRVDIMGAVI SVKERETFYS YGVDDATGVI NCVCWKKLSN AESSSDPAIL STARELSMTS QLKKLQETIE QKTRIGIGDI IRVRGSVRMF REEREICANI YYKVDDPVWN MQIARMLELP KLYQKVYDQP FRNPALQEEE ALNNKDNLDL AGLTSLLSEK IKEFLQEKKM QSFYQQELET VESLQSLASR PVTHSTGSDQ VELKDSGTSG VAQRVFKNAL QLLQEKGLVF QRDSGSDKLY YVTTKDKDLQ QKIYHIIKED CQKPNHMEKG CHLLHILNCV HLNLRWDLSK AVLQRVLELL EDQSDIVSTA DHYYAAF
3D Structure
ModBase 3D Structure for Q8K2X3
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Obfc1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for Obfc1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_780569.1
[Other Products]
NCBI GenBank Nucleotide #
NM_175360.2
[Other Products]
UniProt Primary Accession #
Q8K2X3
[Other Products]
UniProt Secondary Accession #
Q3TPH0; Q8C6I6[Other Products]
UniProt Related Accession #
Q8K2X3[Other Products]
Molecular Weight
43,486 Da
NCBI Official Full Name
CST complex subunit STN1
NCBI Official Synonym Full Names
STN1, CST complex subunit
NCBI Official Symbol
Stn1??[Similar Products]
NCBI Official Synonym Symbols
AAF44; Obfc1; AAF-44; RPA-32; AI413458; 0610009H20Rik; 2310057J23Rik
??[Similar Products]
NCBI Protein Information
CST complex subunit STN1
UniProt Protein Name
CST complex subunit STN1
UniProt Synonym Protein Names
Alpha-accessory factor of 44 kDa; AAF-44; AAF44; Oligonucleotide/oligosaccharide-binding fold-containing protein 1; Suppressor of cdc thirteen homolog
Protein Family
CST complex
UniProt Gene Name
Stn1??[Similar Products]
UniProt Synonym Gene Names
AAF-44; AAF44??[Similar Products]
UniProt Comments for Obfc1
Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22748632). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres. Promotes general replication start following replication-fork stalling implicating new origin firing. May be in involved in C-strand fill-in during late S/G2 phase independent of its role in telomere duplex replication ().
Research Articles on Obfc1
1. OBFC1 is identical to the previously described 44 kDa subunit of DNA-pol-alpha/primase associated factor (AAF) which increases polymerase-alpha/primase template affinity and stimulate both DNA primase and polymerase-alpha activities in vitro.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
