Neuroendocrine convertase 1; proprotein convertase subtilisin/kexin type 1; BDP antibody; EC antibody; NEC 1 antibody; NEC1 antibody; NEC1_HUMAN antibody; Neuroendocrine convertase 1 antibody; PC1 antibody; PC3 antibody; PCSK1 antibody; Prohormone convertase 1 antibody; Prohormone convertase 1/3 antibody; Prohormone convertase 3 antibody; Proprotein convertase 1 antibody; SPC3 antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Immunogen affinity purified.

Lyophilized

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-PCSK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

PCSK1(Proprotein Convertase, Subtilisin/Kexin-Type, 1), also known as PC1 or NEC1, is an enzyme that in humans is encoded by the PCSK1 gene. Proprotein convertase-1 is a neuroendocrine convertase that belongs to a family of subtilisin-like serine endoproteases that process large precursor proteins into mature bioactive products. By in situ hybridization, Seidah et al.(1991) mapped the NEC1 gene to human chromosome 5q15-q21 and mouse chromosome 13. Ohagi et al.(1996) noted that PC1 initiates the sequential processing of proinsulin to insulin by cleaving the proinsulin molecule on the C-terminal side of the dibasic peptide, arg31-arg32, joining the B-chain and C-peptide. By observing the phenotypic features in patients with PC1 mutations, Jackson et al.(2003) concluded that human intestinal absorptive function is dependent on PC1 activity.

Western Blot (WB), Immunohistochemistry (IHC) - Paraffin-embedded Section, Immunofluroescence (IF)

Western Blot:
Concentration: 0.1-0.5 ug/ml
Tested Species: Human, Rat
Predicted Species: Mouse
Antigen Retrieval: N/A

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1 ug/ml
Tested Species: Rat
Predicted Species: Human, Mouse
Antigen Retrieval: By Heat

Immunofluorescence:
Concentration: 2 ug/ml
Tested Species: Human
Predicted Species: N/A
Antigen Retrieval: N/A

WB: The detection limit for PCSK1 is approximately 1ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling paraffin section in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Other applications have not been tested.
Optimal dilutions should be determined by end users.

Figure 1. Western blot analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: A549 Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: PANC Cell Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC1/3/PCSK1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC1/3/PCSK1 at approximately 84KD. The expected band size for PC1/3/PCSK1 is at 84KD.

Figure 2. IHC analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody
PC1/3/PCSK1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Figure 3. IF analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody
PC1/3/PCSK1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-PC1/3/PCSK1 Antibody overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight 488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

proprotein convertase subtilisin/kexin type 1

neuroendocrine convertase 1; prohormone convertase 1; prohormone convertase 3

Neuroendocrine convertase 1

NEC1; NEC 1; PC1??[Similar Products]

NEC1_HUMAN

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to subcellular compartments where a second autocatalytic even takes place and the catalytic activity is acquired. The protease is packaged into and activated in dense core secretory granules and expressed in the neuroendocrine system and brain. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It functions in the proteolytic activation of polypeptide hormones and neuropeptides precursors. Mutations in this gene have been associated with susceptibility to obesity and proprotein convertase 1/3 deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene [provided by RefSeq, Jan 2014]

PCSK1: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: EC 3.4.21.93; Protease

Chromosomal Location of Human Ortholog: 5q15-q21

Cellular Component: Golgi apparatus; extracellular space; transport vesicle

Molecular Function: serine-type endopeptidase activity

Biological Process: cellular protein metabolic process; cell-cell signaling; metabolic process; peptide hormone processing; proteolysis; regulation of insulin secretion; peptide biosynthetic process

Disease: Proprotein Convertase 1/3 Deficiency; Body Mass Index Quantitative Trait Locus 12

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