For Research Use Only. Not for use in diagnostic procedures.

At -80 degree C
Shelf Life: 12 months

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Product Information: Acid Sphingomyelinase Activity Colorimetric Assay Kit: Convenient assay to measure ASMase Activity in tissues, cells & serum. High-Throughput adaptable. Detection limit: as low as 3 mU/ml. 100 assays.

Background: Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. MyBioSource's Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.

Summary: Detection method- Absorbance (570 nm)
Applications - Measurement of ASMase activity in various tissues/cell extracts - Screening of ASMase inhibitors

Metabolism Assays; Lipids Metabolism; Metabolism Assays; Metabolism Assays (A-Z)

Measurement of ASMase activity in various tissues/cell extracts
Screening of ASMase inhibitors

69,624 Da[Similar Products]

sphingomyelin phosphodiesterase 1, acid lysosomal

sphingomyelin phosphodiesterase

Sphingomyelin phosphodiesterase

ASM; aSMase??[Similar Products]

ASM_HUMAN

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.12; Phosphodiesterase; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 11p15.4-p15.1

Cellular Component: extracellular space; lysosomal lumen; lysosome; plasma membrane; endosome; lamellar body

Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds

Biological Process: response to drug; nervous system development; negative regulation of MAP kinase activity; sphingolipid metabolic process; sphingomyelin metabolic process; positive regulation of apoptosis; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process; response to cocaine; signal transduction; positive regulation of protein amino acid dephosphorylation

Disease: Niemann-pick Disease, Type B; Niemann-pick Disease, Type A

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