Full Product Name
OCA2 siRNA (Mouse)
Product Synonym Names
P; P protein; Melanocyte-specific transporter protein; Pink-eyed dilution protein
Product Gene Name
OCA2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q62052
Specificity
OCA2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse OCA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of OCA2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
OCA2 sirna
siRNA to inhibit OCA2 expression using RNA interference
Applications Tested/Suitable for OCA2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for OCA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_068679.1
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NCBI GenBank Nucleotide #
NM_021879.2
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UniProt Primary Accession #
Q62052
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UniProt Secondary Accession #
Q0VBP9[Other Products]
UniProt Related Accession #
Q62052[Other Products]
Molecular Weight
91,869 Da
NCBI Official Full Name
P protein
NCBI Official Synonym Full Names
oculocutaneous albinism II
NCBI Official Symbol
Oca2??[Similar Products]
NCBI Official Synonym Symbols
p; D7Nic1; D7H15S12; D7Icr28RN
??[Similar Products]
NCBI Protein Information
P protein
UniProt Protein Name
P protein
UniProt Synonym Protein Names
Melanocyte-specific transporter protein; Pink-eyed dilution protein
UniProt Gene Name
Oca2??[Similar Products]
UniProt Synonym Gene Names
P??[Similar Products]
UniProt Entry Name
P_MOUSE
UniProt Comments for OCA2
OCA2: a transporer of tyrosine, a precursor for melanin synthesis, within melanocytes. Regulates the pH of melanosomes and melanosome maturation. One of the components of the mammalian pigmentary system. Seems to regulate the postranslational processing of tyrosinase, which catalyzes the limiting reaction in melanin synthesis. May serve as a key control point at which ethnic skin color variation is determined. Major determinant of brown and/or blue eye color. Belongs to the SLC13A transporter family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter
Cellular Component: endoplasmic reticulum membrane; membrane; lysosomal membrane; melanosome membrane; integral to membrane; endosome membrane
Biological Process: melanin biosynthetic process; cell proliferation; pigmentation; transport; pigmentation during development; melanocyte differentiation; transmembrane transport; spermatid development
Research Articles on OCA2
1. High-resolution array-CGH in patients with oculocutaneous albinism identifies new deletions of the TYR, OCA2, and SLC45A2 genes and a complex rearrangement of the OCA2 gene.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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