Full Product Name
OCA2 siRNA (Human)
Product Synonym Names
D15S12; P; P protein; Melanocyte-specific transporter protein; Pink-eyed dilution protein homolog
Product Gene Name
OCA2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q04671
Specificity
OCA2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human OCA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of OCA2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
OCA2 sirna
siRNA to inhibit OCA2 expression using RNA interference
Applications Tested/Suitable for OCA2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for OCA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000266.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000275.2
[Other Products]
UniProt Primary Accession #
Q04671
[Other Products]
UniProt Secondary Accession #
Q15211; Q15212; Q96EN1; Q9UMI5[Other Products]
UniProt Related Accession #
Q04671[Other Products]
Molecular Weight
74,312 Da
NCBI Official Full Name
P protein isoform 1
NCBI Official Synonym Full Names
oculocutaneous albinism II
NCBI Official Symbol
OCA2??[Similar Products]
NCBI Official Synonym Symbols
P; BEY; PED; BEY1; BEY2; BOCA; EYCL; HCL3; EYCL2; EYCL3; SHEP1; D15S12
??[Similar Products]
NCBI Protein Information
P protein
UniProt Protein Name
P protein
UniProt Synonym Protein Names
Melanocyte-specific transporter protein; Pink-eyed dilution protein homolog
UniProt Gene Name
OCA2??[Similar Products]
UniProt Synonym Gene Names
D15S12; P??[Similar Products]
UniProt Entry Name
P_HUMAN
NCBI Summary for OCA2
This gene encodes the human homolog of the mouse p (pink-eyed dilution) gene. The encoded protein is believed to be an integral membrane protein involved in small molecule transport, specifically tyrosine, which is a precursor to melanin synthesis. It is involved in mammalian pigmentation, where it may control skin color variation and act as a determinant of brown or blue eye color. Mutations in this gene result in type 2 oculocutaneous albinism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Comments for OCA2
OCA2: a transporer of tyrosine, a precursor for melanin synthesis, within melanocytes. Regulates the pH of melanosomes and melanosome maturation. One of the components of the mammalian pigmentary system. Seems to regulate the postranslational processing of tyrosinase, which catalyzes the limiting reaction in melanin synthesis. May serve as a key control point at which ethnic skin color variation is determined. Major determinant of brown and/or blue eye color. Belongs to the SLC13A transporter family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter
Chromosomal Location of Human Ortholog: 15q
Cellular Component: endoplasmic reticulum membrane; lysosomal membrane; cytoplasm; melanosome membrane; integral to membrane; endosome membrane
Molecular Function: protein binding; transporter activity; L-tyrosine transmembrane transporter activity
Biological Process: melanin biosynthetic process; cell proliferation; tyrosine transport; melanocyte differentiation; eye pigment biosynthetic process; transmembrane transport; spermatid development
Disease: Albinism, Oculocutaneous, Type Ii; Skin/hair/eye Pigmentation, Variation In, 1
Research Articles on OCA2
1. individuals from ''El Santuario and Marinilla, Antioquia'', genetically isolated northwestern towns in Colombia well known for its high albinism prevalence [7], were all homozygous for the previously reported p.A787T mutation in OCA2 gene
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
