Claudin-16; Paracellin-1; PCLN-1; CLDN16; PCLN1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This CLDN16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-33 amino acids from the N-terminal region of human CLDN16.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.36 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CLDN16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Tight junctions represent one mode of cell-to-cell
adhesion in epithelial or endothelial cell sheets, forming
continuous seals around cells and serving as a physical barrier to
prevent solutes and water from passing freely through the
paracellular space. These junctions are comprised of sets of
continuous networking strands in the outwardly facing cytoplasmic
leaflet, with complementary grooves in the inwardly facing
extracytoplasmic leaflet. The protein encoded by this gene, a
member of the claudin family, is an integral membrane protein and a
component of tight junction strands. It is found primarily in the
kidneys, specifically in the thick ascending limb of Henle, where
it acts as either an intercellular pore or ion concentration sensor
to regulate the paracellular resorption of magnesium ions. Defects
in this gene are a cause of primary hypomagnesemia, which is
characterized by massive renal magnesium wasting with
hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis
and renal failure. This gene and the CLDN1 gene are clustered on
chromosome 3q28.

Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

WB~~1:1000

CLDN16 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CLDN16 antibody detected the CLDN16 protein (arrow).

CLDN16 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human Kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLDN16 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CLDN16 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

33836

claudin 16

claudin-16

Claudin-16

PCLN1; PCLN-1??[Similar Products]

CLD16_HUMAN

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28. [provided by RefSeq, Jun 2010]

Claudin-16: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Defects in CLDN16 are the cause of hypomagnesemia type 3 (HOMG3); also known as familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC). HOMG3 is a progressive renal disease characterized by primary renal magnesium wasting with hypomagnesemia, hypercalciuria and nephrocalcinosis. Recurrent urinary tract infections and kidney stones are often observed. In spite of hypercalciuria, patients do not show hypocalcemia. Belongs to the claudin family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: tight junction; plasma membrane; integral to membrane

Molecular Function: identical protein binding; protein binding; structural molecule activity; magnesium ion transmembrane transporter activity

Biological Process: cellular metal ion homeostasis; excretion; calcium-independent cell-cell adhesion; magnesium ion transport

Disease: Hypomagnesemia 3, Renal

Kuo, S.J., et al. Oncol. Rep. 24(3):759-766(2010)
Efrati, E., et al. Cell. Physiol. Biochem. 25(6):705-714(2010)
Shuen, A.Y., et al. Clin. Chim. Acta 409 (1-2), 28-32 (2009) :
Al-Haggar, M., et al. Clin. Exp. Nephrol. 13(4):288-294(2009)
Lal-Nag, M., et al. Genome Biol. 10 (8), 235 (2009) :

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