Rabbit Polyclonal to Human CLDN16 / Claudin 16; Human CLDN16 / Claudin 16; Claudin 16; Claudin-16; Paracellin-1; PCLN1; PCLN-1; HOMG3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Human Claudin 16 / Paracellin-1

Immunoaffinity purified

PBS, 0.09% sodium azide

Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.

Small volumes of anti-CLDN16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CLDN16 Antibody, Claudin 16 Antibody, Claudin-16 Antibody, Paracellin-1 Antibody, PCLN1 Antibody, PCLN-1 Antibody, HOMG3 Antibody Description: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet.

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)

Flow Cytometry (1:10 - 1:50)
IHC-P (1:100)
WB (1:1000)

Anti-CLDN16 / Claudin 16 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-CLDN16 / Claudin 16 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

CLDN16 Antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The CLDN16 antibody detected the CLDN16 protein (arrow).

CLDN16 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

33,836 Da

claudin 16

claudin-16

Claudin-16

PCLN1; PCLN-1??[Similar Products]

CLD16_HUMAN

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28. [provided by RefSeq, Jun 2010]

Claudin-16: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Defects in CLDN16 are the cause of hypomagnesemia type 3 (HOMG3); also known as familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC). HOMG3 is a progressive renal disease characterized by primary renal magnesium wasting with hypomagnesemia, hypercalciuria and nephrocalcinosis. Recurrent urinary tract infections and kidney stones are often observed. In spite of hypercalciuria, patients do not show hypocalcemia. Belongs to the claudin family.

Protein type: Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: integral to membrane; plasma membrane; tight junction

Molecular Function: identical protein binding; magnesium ion transmembrane transporter activity; protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion; cellular metal ion homeostasis; excretion; ion transport; positive regulation of calcium ion transport

Disease: Hypomagnesemia 3, Renal

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