For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

23C6

Mouse

Purified
Purified IgG - liquid

IgG concentration 1.0 mg/ml (lot specific)

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch

Small volumes of anti-CD51/CD61 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse anti Human CD51/CD61 antibody, clone 23C6 recognizes the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronectin at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Mouse anti Human CD51/CD61 antibody, clone 23C6 reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP)

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/40; Maximum Dilution: 1/80

Staining of KG1a cell line with Mouse anti Human CD51/CD61:RPE (MBS213878)

86,694 Da

integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)

integrin beta-3; platelet membrane glycoprotein IIIa

Integrin beta-3

GP3A; GPIIIa??[Similar Products]

ITB3_HUMAN

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: filopodium membrane; platelet alpha granule membrane; cell surface; focal adhesion; integral to plasma membrane; microvillus membrane; melanosome; plasma membrane; nucleus; integrin complex; receptor complex

Molecular Function: identical protein binding; protein binding; protease binding; extracellular matrix binding; vascular endothelial growth factor receptor 2 binding; fibronectin binding; protein disulfide isomerase activity; platelet-derived growth factor receptor binding; receptor activity; cell adhesion molecule binding

Biological Process: negative regulation of lipid transport; axon guidance; extracellular matrix organization and biogenesis; entry of virus into host cell; wound healing; protein folding; cell-matrix adhesion; smooth muscle cell migration; negative chemotaxis; mesodermal cell differentiation; positive regulation of vascular endothelial growth factor receptor signaling pathway; platelet degranulation; cell-substrate junction assembly; cell growth; cell adhesion; integrin-mediated signaling pathway; platelet activation; cell migration; regulation of bone resorption; negative regulation of lipoprotein metabolic process; angiogenesis involved in wound healing; cell-substrate adhesion; heterotypic cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; activation of protein kinase activity; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; tube development; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration

Disease: Myocardial Infarction, Susceptibility To; Glanzmann Thrombasthenia; Bleeding Disorder, Platelet-type, 16

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