Full Product Name
HAMSTER ANTI MOUSE CD61:RPE
Product Gene Name
anti-CD61 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O54890
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitution
Reconstitute with 1.0 ml distilled water
Perservative Stabilisers
Immunogen
Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4
Fusion Partners
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of anti-CD61 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD61 antibody
Hamster anti Mouse CD61 antibody, clone HM beta 3-1 recognizes the murine integrin beta 3 subunit (CD61), a 90kDa a type I membrane protein, which is expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively. The heterodimers formed with CD61 are receptor for a variety of ligands including fibrinogen, fibronectin, von Willebrands factor (vWF), vitronectin and thrombospondin. Hamster anti Mouse CD61 antibody, clone HM beta 3-1 is reported to partially inhibit the binding of CD61 to fibronectin (Yasuda et al. 1995).
Applications Tested/Suitable for anti-CD61 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD61 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
Testing Data of anti-CD61 antibody
Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:RPE
Testing Data of anti-CD61 antibody
Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:Alexa Fluor 647 (MBS212674A647)
Testing Data of anti-CD61 antibody
Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:FITC (MBS210936)
Testing Data of anti-CD61 antibody
Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:Alexa Fluor ?488 (MBS212674A488)
Testing Data of anti-CD61 antibody
Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:Biotin (MBS210443)
Testing Data of anti-CD61 antibody
Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:FITC (MBS210935)
NCBI/Uniprot data below describe general gene information for CD61. It may not necessarily be applicable to this product.
NCBI Accession #
NP_058060.2
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NCBI GenBank Nucleotide #
NM_016780.2
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UniProt Primary Accession #
O54890
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UniProt Secondary Accession #
Q3TZC6[Other Products]
UniProt Related Accession #
O54890[Other Products]
Molecular Weight
86,738 Da
NCBI Official Full Name
integrin beta-3
NCBI Official Synonym Full Names
integrin beta 3
NCBI Official Symbol
Itgb3??[Similar Products]
NCBI Official Synonym Symbols
CD61; GP3A; INGRB3
??[Similar Products]
NCBI Protein Information
integrin beta-3; GPIIIa; platelet gpIIIa; platelet membrane glycoprotein IIIa
UniProt Protein Name
Integrin beta-3
UniProt Synonym Protein Names
Platelet membrane glycoprotein IIIa; GPIIIa; CD_antigen: CD61
UniProt Gene Name
Itgb3??[Similar Products]
UniProt Synonym Gene Names
GPIIIa??[Similar Products]
UniProt Entry Name
ITB3_MOUSE
UniProt Comments for CD61
ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion
Cellular Component: filopodium membrane; cell surface; focal adhesion; integral to membrane; cell projection; membrane; microvillus membrane; apical plasma membrane; plasma membrane; melanosome; cell junction; integrin complex; nucleus; receptor complex; external side of plasma membrane
Molecular Function: integrin binding; identical protein binding; protein binding; protease binding; extracellular matrix binding; vascular endothelial growth factor receptor 2 binding; fibronectin binding; protein disulfide isomerase activity; receptor activity; cell adhesion molecule binding; peptide binding
Biological Process: negative regulation of lipid transport; entry of virus into host cell; protein folding; positive regulation of osteoclast differentiation; positive regulation of leukocyte migration; positive regulation of smooth muscle cell proliferation; multicellular organismal development; cell-matrix adhesion; smooth muscle cell migration; negative chemotaxis; positive regulation of smooth muscle cell migration; regulation of cell migration; positive regulation of MAPKKK cascade; cell-substrate junction assembly; positive regulation of bone resorption; cell adhesion; cell growth; integrin-mediated signaling pathway; platelet activation; cell migration; negative regulation of lipoprotein metabolic process; positive regulation of cell adhesion mediated by integrin; cell projection morphogenesis; angiogenesis involved in wound healing; regulation of release of sequestered calcium ion into cytosol; cell-substrate adhesion; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of osteoblast proliferation; activation of protein kinase activity; positive regulation of endothelial cell proliferation; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of cell migration
Research Articles on CD61
1. in the presence of both Itgb3 and Slc6a4 heterozygozity, 5-HT uptake was similar to wild-type levels, revealing a significant Slc6a4 by Itgb3 genetic interaction
Precautions
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