ABP1; HIP-55; SH3P7; src homology 3 domain-containing protein HIP-55

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse DBNL. No significant cross-reactivity or interference between Mouse DBNL and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of DBNL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285412 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Drebrin (DBNL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DBNL. The ELISA analytical biochemical technique of the MBS285412 kit is based on DBNL antibody-DBNL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DBNL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DBNL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DBNL in samples. An antibody specific for DBNL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDBNL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DBNL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DBNL bound in the initial step. The color development is stopped and the intensity of the color is measured.

48,341 Da

drebrin-like

drebrin-like protein

Drebrin-like protein

DBNL: an SH3-containing adaptor protein. Interacts with hematopoietic progenitor kinase 1a (HIPK1). It is recruited to glycolipid-enriched microdomains following T cell receptor (TCR) stimulation. Interacts with and is phosphorylated by ZAP-70 after TCR signaling. May regulate JNK activation following TCR signaling, presumably via its interaction with HIPKIt is a member of the drebrin family of proteins that are developmentally regulated in the brain.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11 A1|11 3.87 cM

Cellular Component: cell cortex; cell junction; cell projection; cytoplasm; cytoplasmic vesicle; cytoskeleton; dendrite; endosome; extracellular exosome; Golgi apparatus; intracellular; lamellipodium; membrane; plasma membrane; podosome; postsynaptic density; ruffle; synapse

Molecular Function: actin binding; actin filament binding; cadherin binding; protein binding; protein C-terminus binding; protein domain specific binding

Biological Process: actin filament bundle formation; actin filament severing; adaptive immune response; barbed-end actin filament capping; endocytosis; immune system process; neuron projection morphogenesis; podosome assembly; Rac protein signal transduction; receptor-mediated endocytosis; synaptogenesis; transport

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