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Drebrin Like Protein (DBNL), ELISA Kit

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產(chǎn)品名稱: Drebrin Like Protein (DBNL), ELISA Kit
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Drebrin Like Protein (DBNL), ELISA Kit


Drebrin Like Protein (DBNL), ELISA Kit  的詳細(xì)介紹
Product Name

Drebrin Like Protein (DBNL), ELISA Kit

Full Product Name

Human Drebrin Like Protein (DBNL) ELISA Kit

Product Synonym Names
ABP1; HIP-55; SH3P7; CMAP; Cervical mucin-associated protein; Drebrin-F; HPK1-interacting protein of 55 kDa; SH3 domain-containing protein 7
Product Gene Name

DBNL elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
Request Current Manual
OMIM
AF197060 mRNA
3D Structure
ModBase 3D Structure for Q9UJU6
Species Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Drebrin Like Protein (DBNL).
No significant cross-reactivity or interference between Drebrin Like Protein (DBNL) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
1.56-100ng/mL
Sensitivity
< 0.63ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Drebrin Like Protein (DBNL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Drebrin Like Protein (DBNL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of DBNL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DBNL purchase
MBS2706936 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Drebrin Like Protein (DBNL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DBNL. The ELISA analytical biochemical technique of the MBS2706936 kit is based on DBNL antibody-DBNL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DBNL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DBNL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for
DBNL elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Drebrin Like Protein (DBNL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Drebrin Like Protein (DBNL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Drebrin Like Protein (DBNL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Drebrin Like Protein (DBNL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of DBNL elisa kit
DBNL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for DBNL. It may not necessarily be applicable to this product.
NCBI GI #
62198235
NCBI GeneID
28988
NCBI Accession #
NP_001014436.1 [Other Products]
NCBI GenBank Nucleotide #
NM_001014436.2 [Other Products]
UniProt Primary Accession #
Q9UJU6 [Other Products]
UniProt Secondary Accession #
P84070; Q6IAI8; Q96F30; Q96K74; Q9HBN8; Q9NR72; A4D2I9; B4DDP6; B4DEM2; C9J7P1[Other Products]
UniProt Related Accession #
Q9UJU6[Other Products]
Molecular Weight
43,041 Da
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NCBI Official Full Name
drebrin-like protein isoform b
NCBI Official Synonym Full Names
drebrin like
NCBI Official Symbol
DBNL??[Similar Products]
NCBI Official Synonym Symbols
ABP1; HIP55; SH3P7; HIP-55
??[Similar Products]
NCBI Protein Information
drebrin-like protein
UniProt Protein Name
Drebrin-like protein
UniProt Synonym Protein Names
Cervical SH3P7; Cervical mucin-associated protein; Drebrin-F; HPK1-interacting protein of 55 kDa; HIP-55; SH3 domain-containing protein 7
Protein Family
Drebrin-like protein
UniProt Gene Name
DBNL??[Similar Products]
UniProt Synonym Gene Names
CMAP; SH3P7; HIP-55??[Similar Products]
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UniProt Comments for DBNL
Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (). May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.
Research Articles on DBNL
1. findings show that mAbp1 and FHL2 are novel binding partners that differentially regulate Rho GTPase signaling and MTLn3 breast cancer cell invasion
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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