CISD2; ERIS; WFS2; ZCD2; NAF-1; Miner1; CDGSH2; ERIS; CDGSH iron-sulfur domain-containing protein 2; Endoplasmic reticulum intermembrane small protein; CDGSH iron sulfur domain 2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

CISD2 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

CISD2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Small volumes of anti-CISD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CISD2 Antibody: Defects in the CISD2 (CDGSH iron sulfur domain 2) gene are a cause of the neurodegenerative disorder Wolfram syndrome 2. CISD2 is a zinc finger protein that localizes to the endoplasmic reticulum and mitochondria and binds an iron/sulfur cluster. CISD2 interacts with Bcl-2 and can be displaced by the BH3-only protein BIK and contributes to the regulation of BIK-initiated autophagy. CISD2 deficiency in mice causes mitochondrial breakdown accompanied by autophagic cell death as well as the development of premature aging phenotype.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2.

Western blot analysis of CISD2 in rat brain tissue lysate with CISD2 antibody at (A) 1 and (B) 2 μg/m

Immunohistochemistry of CISD2 in rat brain tissue with CISD2 antibody at 2.5 μg/ml.

Immunofluorescence of CISD2 in rat brain tissue with CISD2 antibody at 20 μg/ml.

Predicted: 15 kDa

Observed: 15 kDa

CDGSH iron sulfur domain 2

CDGSH iron-sulfur domain-containing protein 2; mitoNEET-related 1 protein; zinc finger, CDGSH-type domain 2; nutrient-deprivation autophagy factor-1; endoplasmic reticulum intermembrane small protein

CDGSH iron-sulfur domain-containing protein 2

CDGSH2; ERIS; ZCD2; Miner1; NAF-1??[Similar Products]

CISD2_HUMAN

The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2. [provided by RefSeq, Mar 2011]

CISD2: a single-pass membrane protein of the endoplasmic reticulum that regulates autophagy. Has also been reported to localize to the mitochondrion outer membrane. Antagonizes ATG6-mediated cellular autophagy. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca2+ stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy. Directly interacts with BCL2; the interaction is disrupted by BIK interaction with BCL2. It has been reported to mainly localizes to the mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome 2 (WFS2), is a rare autosomal recessive disorder characterized by optic atrophy and diabetes mellitus. Other symptoms include the presence of profound upper gastrointestinal ulceration, significant bleeding tendency, defective platelet aggregation with collagen and various neurological symptoms. Initially thought to be a zinc-finger protein, it was later shown to bind 1 2Fe-2S cluster instead.

Protein type: Autophagy; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: mitochondrial outer membrane; endoplasmic reticulum membrane; protein complex; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: 2 iron, 2 sulfur cluster binding; protein binding; protein homodimerization activity; metal ion binding

Biological Process: mitochondrion degradation; multicellular organismal aging; regulation of autophagy

Disease: Wolfram Syndrome 2

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