Product Name
PPP1R9B, Polyclonal Antibody
Full Product Name
PPP1R9B Polyclonal Antibody
Product Synonym Names
Spn; SPINO; PPP1R6; PPP1R9
Product Gene Name
anti-PPP1R9B antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96SB3
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Immunogen
A synthetic peptide of human PPP1R9B
Calculated Molecular Weight
89kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-PPP1R9B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PPP1R9B antibody
Spinophilin is a regulatory subunit of protein phosphatase-1 catalytic subunit (PP1; see MIM 176875) and is highly enriched in dendritic spines, specialized protrusions from dendritic shafts that receive most of the excitatory input in the central nervous system (Allen et al., 1997)
Product Categories/Family for anti-PPP1R9B antibody
Polyclonal
Applications Tested/Suitable for anti-PPP1R9B antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-PPP1R9B antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:20 - 1:50
Western Blot (WB) of anti-PPP1R9B antibody
Western blot analysis of extracts of various cell lines, using PPP1R9B antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
NCBI/Uniprot data below describe general gene information for PPP1R9B. It may not necessarily be applicable to this product.
NCBI Accession #
Q96SB3.2
[Other Products]
UniProt Primary Accession #
Q96SB3
[Other Products]
UniProt Secondary Accession #
Q8TCR9[Other Products]
UniProt Related Accession #
Q96SB3[Other Products]
NCBI Official Full Name
Neurabin-2
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 9B
NCBI Official Symbol
PPP1R9B??[Similar Products]
NCBI Official Synonym Symbols
Spn; SPINO; PPP1R6; PPP1R9
??[Similar Products]
NCBI Protein Information
neurabin-2; neurabin-II; protein phosphatase 1, regulatory (inhibitor) subunit 9B; protein phosphatase 1, regulatory subunit 9B, spinophilin
UniProt Protein Name
Neurabin-2
UniProt Synonym Protein Names
Neurabin-II; Protein phosphatase 1 regulatory subunit 9B; Spinophilin
UniProt Gene Name
PPP1R9B??[Similar Products]
UniProt Synonym Gene Names
PPP1R6??[Similar Products]
UniProt Entry Name
NEB2_HUMAN
NCBI Summary for PPP1R9B
This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors. [provided by RefSeq, Feb 2014]
UniProt Comments for PPP1R9B
Spinophilin: actin-binding regulatory subunit of protein phosphatase 1 (PP1). The actin-binding domain of spinophilin is necessary and sufficient for targeting of spinophilin to dendrites and dendritic spines. May play a role in establishing a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Interacts with p14-ARF. Contains one PDZ/DHR domain.
Protein type: Protein phosphatase, regulatory subunit; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: nucleoplasm; cortical actin cytoskeleton; adherens junction; lamellipodium; cytoplasm; dendritic spine; plasma membrane; synapse; protein phosphatase type 1 complex; filopodium
Molecular Function: protein binding; protein phosphatase inhibitor activity; actin binding; protein phosphatase 1 binding
Biological Process: regulation of synaptic transmission; regulation of protein amino acid phosphorylation; cell migration; regulation of cell growth by extracellular stimulus; filopodium formation; RNA splicing; regulation of exit from mitosis; calcium-mediated signaling; actin filament organization; regulation of cell proliferation; negative regulation of catalytic activity; dendrite development; negative regulation of cell growth; cell cycle arrest
Research Articles on PPP1R9B
1. spinophilin might play a previously unrecognized role in the pathogenesis of head and neck squamous cell carcinoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
