Product Name
PPP1R9B, siRNA
Full Product Name
PPP1R9B siRNA (Human)
Product Synonym Names
PPP1R6; Neurabin-2; Neurabin-II; Protein phosphatase 1 regulatory subunit 9B; Spinophilin
Product Gene Name
PPP1R9B sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q96SB3
Specificity
PPP1R9B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PPP1R9B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PPP1R9B sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PPP1R9B sirna
siRNA to inhibit PPP1R9B expression using RNA interference
Applications Tested/Suitable for PPP1R9B sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PPP1R9B. It may not necessarily be applicable to this product.
NCBI Accession #
NP_115984.3
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NCBI GenBank Nucleotide #
NM_032595.4
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UniProt Primary Accession #
Q96SB3
[Other Products]
UniProt Secondary Accession #
Q8TCR9[Other Products]
UniProt Related Accession #
Q96SB3[Other Products]
Molecular Weight
89,192 Da
NCBI Official Full Name
neurabin-2
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 9B
NCBI Official Symbol
PPP1R9B??[Similar Products]
NCBI Official Synonym Symbols
Spn; SPINO; PPP1R6; PPP1R9
??[Similar Products]
NCBI Protein Information
neurabin-2
UniProt Protein Name
Neurabin-2
UniProt Synonym Protein Names
Neurabin-II; Protein phosphatase 1 regulatory subunit 9B; Spinophilin
UniProt Gene Name
PPP1R9B??[Similar Products]
UniProt Synonym Gene Names
PPP1R6??[Similar Products]
UniProt Entry Name
NEB2_HUMAN
NCBI Summary for PPP1R9B
This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors. [provided by RefSeq, Feb 2014]
UniProt Comments for PPP1R9B
Spinophilin: actin-binding regulatory subunit of protein phosphatase 1 (PP1). The actin-binding domain of spinophilin is necessary and sufficient for targeting of spinophilin to dendrites and dendritic spines. May play a role in establishing a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Interacts with p14-ARF. Contains one PDZ/DHR domain.
Protein type: Adaptor/scaffold; Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: nucleoplasm; cortical actin cytoskeleton; adherens junction; lamellipodium; cytoplasm; dendritic spine; plasma membrane; synapse; protein phosphatase type 1 complex; filopodium
Molecular Function: protein binding; protein phosphatase inhibitor activity; actin binding; protein phosphatase 1 binding
Biological Process: regulation of synaptic transmission; cell migration; regulation of cell growth by extracellular stimulus; regulation of protein amino acid phosphorylation; filopodium formation; RNA splicing; calcium-mediated signaling; regulation of exit from mitosis; actin filament organization; regulation of cell proliferation; negative regulation of catalytic activity; dendrite development; negative regulation of cell growth; cell cycle arrest
Research Articles on PPP1R9B
1. Colorectal carcinoma expression of spinophilin expression determines cellular growth, cancer stemness and 5-flourouracil resistance.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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