Full Product Name
BAGE siRNA (Human)
Product Synonym Names
BAGE1; B melanoma antigen 1; B melanoma antigen; Antigen MZ2-BA; Cancer/testis antigen 2.1; CT2.1
Product Gene Name
BAGE sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13072
Specificity
BAGE siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BAGE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of BAGE sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BAGE sirna
siRNA to inhibit BAGE expression using RNA interference
Applications Tested/Suitable for BAGE sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for BAGE. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001178.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001187.1
[Other Products]
UniProt Primary Accession #
Q13072
[Other Products]
UniProt Secondary Accession #
A4IF47[Other Products]
UniProt Related Accession #
Q13072[Other Products]
Molecular Weight
4,810 Da
NCBI Official Full Name
B melanoma antigen 1
NCBI Official Synonym Full Names
B melanoma antigen
NCBI Official Symbol
BAGE??[Similar Products]
NCBI Official Synonym Symbols
BAGE1; CT2.1
??[Similar Products]
NCBI Protein Information
B melanoma antigen 1
UniProt Protein Name
B melanoma antigen 1
UniProt Synonym Protein Names
Antigen MZ2-BA; Cancer/testis antigen 2.1; CT2.1
Protein Family
B melanoma antigen
UniProt Gene Name
BAGE??[Similar Products]
UniProt Synonym Gene Names
BAGE1; B melanoma antigen; CT2.1??[Similar Products]
UniProt Entry Name
BAGE1_HUMAN
NCBI Summary for BAGE
This gene encodes a tumor antigen recognized by autologous cytolytic lymphocytes (CTL). [provided by RefSeq, Jul 2008]
UniProt Comments for BAGE
BAGE: Unknown. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Belongs to the BAGE family. 1 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Cancer Testis Antigen (CTA); Secreted
Chromosomal Location of Human Ortholog: 21p11.1 not on reference assembly
Cellular Component: extracellular region
Research Articles on BAGE
1. Tumor-specific antigen BAGE may play a role in the occurrence and development of ovarian cancer and can be used as one of the important indicators for early diagnosis, efficacy evaluation and prognostic determination of ovarian cancer.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
