For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Mouse WRN. No significant cross-reactivity or interference between Mouse WRN and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of WRN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282526 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Werner syndrome ATP-dependent helicase (WRN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WRN. The ELISA analytical biochemical technique of the MBS282526 kit is based on WRN antibody-WRN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WRN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WRN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WRN in samples. An antibody specific for WRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWRN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WRN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WRN bound in the initial step. The color development is stopped and the intensity of the color is measured.

157,204 Da

Werner syndrome RecQ like helicase

Werner syndrome ATP-dependent helicase homolog

Werner syndrome ATP-dependent helicase homolog

WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating s for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.

Protein type: Cell cycle regulation; DNA repair, damage; DNA-binding; EC 3.6.4.12; Helicase; Nucleolus

Chromosomal Location of Human Ortholog: 8 A3|8 20.3 cM

Cellular Component: centrosome; chromosome, telomeric region; cytoplasm; intracellular; MutLalpha complex; neuron projection; nuclear speck; nucleolus; nucleoplasm; nucleus; replication fork

Molecular Function: 3'-5' DNA helicase activity; 3'-5' exonuclease activity; ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; catalytic activity; chromatin binding; DNA binding; DNA helicase activity; exonuclease activity; four-way junction DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; hydrolase activity; magnesium ion binding; manganese ion binding; metal ion binding; nuclease activity; nucleic acid binding; nucleotide binding; protein binding; protein complex binding; protein homodimerization activity; Y-form DNA binding

Biological Process: base-excision repair; cell aging; cellular metabolic process; cellular response to DNA damage stimulus; cellular response to starvation; DNA duplex unwinding; DNA metabolic process; DNA recombination; DNA repair; DNA replication; DNA synthesis involved in DNA repair; double-strand break repair; double-strand break repair via homologous recombination; metabolic process; multicellular organismal aging; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; nucleolus to nucleoplasm transport; positive regulation of hydrolase activity; regulation of apoptosis; regulation of growth rate; replication fork processing; replicative cell aging; response to oxidative stress; response to UV-C; telomere maintenance

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