Full Product Name
WRN siRNA (Mouse)
Product Synonym Names
Werner syndrome ATP-dependent helicase homolog; Exonuclease WRN
Product Gene Name
WRN sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O09053
Specificity
WRN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WRN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of WRN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
WRN sirna
siRNA to inhibit WRN expression using RNA interference
Applications Tested/Suitable for WRN sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for WRN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001116294.1
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NCBI GenBank Nucleotide #
NM_001122822.1
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UniProt Primary Accession #
O09053
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UniProt Secondary Accession #
O09050; Q80YP9; Q9JKD4; Q9Z241; Q9Z242[Other Products]
UniProt Related Accession #
O09053[Other Products]
Molecular Weight
157,204 Da
NCBI Official Full Name
Werner syndrome ATP-dependent helicase homolog
NCBI Official Synonym Full Names
Werner syndrome homolog (human)
NCBI Official Symbol
Wrn??[Similar Products]
NCBI Official Synonym Symbols
AI846146
??[Similar Products]
NCBI Protein Information
Werner syndrome ATP-dependent helicase homolog
UniProt Protein Name
Werner syndrome ATP-dependent helicase homolog
UniProt Synonym Protein Names
Exonuclease WRN (EC:3.1.-.-)
Protein Family
Werner syndrome ATP-dependent helicase
UniProt Gene Name
Wrn??[Similar Products]
UniProt Entry Name
WRN_MOUSE
UniProt Comments for WRN
WRN: Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating s for RP-A. Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer. Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1. Interacts with EXO1, PCNA and SUPV3L1. Belongs to the helicase family. RecQ subfamily.
Protein type: Helicase; DNA repair, damage; DNA-binding; EC 3.6.4.12; Nucleolus; Cell cycle regulation
Cellular Component: MutLalpha complex; nucleoplasm; centrosome; nucleolus; intracellular; nucleus
Molecular Function: G-quadruplex DNA binding; protein homodimerization activity; metal ion binding; ATPase activity; magnesium ion binding; 3'-5' DNA helicase activity; bubble DNA binding; Y-form DNA binding; four-way junction helicase activity; nucleic acid binding; ATP-dependent 3'-5' DNA helicase activity; exonuclease activity; protein complex binding; 3'-5' exonuclease activity; catalytic activity; ATP binding; nuclease activity; hydrolase activity; nucleotide binding; ATP-dependent helicase activity; helicase activity; T/G mismatch-specific endonuclease activity; phosphoric ester hydrolase activity; ATP-dependent DNA helicase activity; protein binding; DNA helicase activity; DNA binding; manganese ion binding
Biological Process: nucleolus to nucleoplasm transport; metabolic process; positive regulation of hydrolase activity; cell aging; multicellular organismal aging; cellular response to starvation; replicative cell aging; DNA repair; response to UV-C; DNA duplex unwinding; DNA recombination; replication fork processing; regulation of apoptosis; cellular metabolic process; DNA synthesis during DNA repair; base-excision repair; double-strand break repair; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; regulation of growth rate; response to oxidative stress; DNA replication; telomere maintenance; response to DNA damage stimulus; DNA metabolic process
Research Articles on WRN
1. The FEN1 E359K germline mutation disrupts the FEN1-WRN interaction and FEN1 GEN activity, causing aneuploidy-associated cancers.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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